PMID- 35945704
OWN - NLM
STAT- MEDLINE
DCOM- 20221110
LR  - 20221219
IS  - 2471-5646 (Electronic)
IS  - 2471-5638 (Linking)
VI  - 337
IP  - 9-10
DP  - 2022 Dec
TI  - Tissue-specific response of the RB-E2F1 complex during mammalian hibernation.
PG  - 1002-1009
LID - 10.1002/jez.2648 [doi]
AB  - Metabolic rate depression during prolonged bouts of torpor is characteristic of 
      mammalian hibernation, reducing energy expenditures over the winter. Cell cycle 
      arrest is observed in quiescent cells during dormancy, partly due to the 
      retinoblastoma (Rb) protein at G(1) /S, given cell division and proliferation are 
      metabolic-costly processes. Rb binds to E2F transcription factors and recruits 
      corepressors (e.g., SUV39H1) to E2F target genes, blocking their transcription 
      and cell cycle passage. Phosphorylation by cyclin-CDK complexes at S780 or S795 
      abolishes Rb-mediated repression, allowing transition into S phase. The present 
      study compares Rb-E2F1 responses between euthermic and torpid states in five 
      organs (brain, heart, kidney, liver, skeletal muscle) of 13-lined ground 
      squirrels (Ictidomys tridecemlineatus). Immunoblotting assessed the expression of 
      Rb, pRb (S780, S795), E2F1, and SUV39H1. Our findings demonstrate multi-tissue 
      upregulation of Rb and SUV39H1 during torpor, with tissue-specific changes to 
      E2F1 and pRb (S780), suggesting Rb-E2F1 contributes to cell cycle control in 
      hibernation.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Singh, Gurjit
AU  - Singh G
AUID- ORCID: 0000-0001-5830-518X
AD  - Department of Biology, Carleton University, Ottawa, Ontario, Canada.
FAU - Bloskie, Tighe
AU  - Bloskie T
AUID- ORCID: 0000-0002-8839-6323
AD  - Department of Biology, Carleton University, Ottawa, Ontario, Canada.
FAU - Storey, Kenneth B
AU  - Storey KB
AUID- ORCID: 0000-0002-7363-1853
AD  - Department of Biology, Carleton University, Ottawa, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220809
PL  - United States
TA  - J Exp Zool A Ecol Integr Physiol
JT  - Journal of experimental zoology. Part A, Ecological and integrative physiology
JID - 101710204
SB  - IM
MH  - Animals
MH  - *Hibernation/physiology
MH  - Sciuridae/physiology
MH  - Phosphorylation
MH  - Muscle, Skeletal/metabolism
MH  - Cell Cycle Checkpoints
OTO - NOTNLM
OT  - cell cycle
OT  - hibernation
OT  - histone methyl transferase
OT  - metabolic rate depression
OT  - phosphorylation
OT  - retinoblastoma protein
OT  - transcription factor
EDAT- 2022/08/11 06:00
MHDA- 2022/11/11 06:00
CRDT- 2022/08/10 00:53
PHST- 2022/07/14 00:00 [revised]
PHST- 2022/03/01 00:00 [received]
PHST- 2022/07/25 00:00 [accepted]
PHST- 2022/08/11 06:00 [pubmed]
PHST- 2022/11/11 06:00 [medline]
PHST- 2022/08/10 00:53 [entrez]
AID - 10.1002/jez.2648 [doi]
PST - ppublish
SO  - J Exp Zool A Ecol Integr Physiol. 2022 Dec;337(9-10):1002-1009. doi: 
      10.1002/jez.2648. Epub 2022 Aug 9.