PMID- 3594571
OWN - NLM
STAT- MEDLINE
DCOM- 19870820
LR  - 20220223
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 50
IP  - 2
DP  - 1987 Jul 17
TI  - Promoter selection in human mitochondria involves binding of a transcription 
      factor to orientation-independent upstream regulatory elements.
PG  - 247-58
AB  - Selective transcription of human mitochondrial DNA requires a transcription 
      factor (mtTF) in addition to an essentially nonselective RNA polymerase. 
      Partially purified mtTF is able to sequester promoter-containing DNA in 
      preinitiation complexes in the absence of mitochondrial RNA polymerase, 
      suggesting a DNA-binding mechanism for factor activity. Functional domains, 
      required for positive transcriptional regulation by mtTF, are identified within 
      both major promoters of human mtDNA through transcription of mutant promoter 
      templates in a reconstituted in vitro system. These domains are essentially 
      coextensive with DNA sequences protected from nuclease digestion by mtTF-binding. 
      Comparison of the sequences of the two mtTF-responsive elements reveals 
      significant homology only when one sequence is inverted; the binding sites are in 
      opposite orientations with respect to the predominant direction of transcription. 
      Thus mtTF may function bidirectionally, requiring additional protein-DNA 
      interactions to dictate transcriptional polarity. The mtTF-responsive elements 
      are arrayed as direct repeats, separated by approximately 80 bp within the 
      displacement-loop region of human mitochondrial DNA; this arrangement may reflect 
      duplication of an ancestral bidirectional promoter, giving rise to separate, 
      unidirectional promoters for each strand.
FAU - Fisher, R P
AU  - Fisher RP
FAU - Topper, J N
AU  - Topper JN
FAU - Clayton, D A
AU  - Clayton DA
LA  - eng
GR  - GM-33088-17/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
SB  - IM
MH  - DNA, Mitochondrial/*genetics
MH  - DNA-Directed RNA Polymerases/metabolism
MH  - *Genes, Regulator
MH  - Humans
MH  - KB Cells
MH  - Mitochondria/metabolism
MH  - *Promoter Regions, Genetic
MH  - Templates, Genetic
MH  - Transcription Factors/*metabolism
MH  - *Transcription, Genetic
EDAT- 1987/07/17 00:00
MHDA- 1987/07/17 00:01
CRDT- 1987/07/17 00:00
PHST- 1987/07/17 00:00 [pubmed]
PHST- 1987/07/17 00:01 [medline]
PHST- 1987/07/17 00:00 [entrez]
AID - 0092-8674(87)90220-0 [pii]
AID - 10.1016/0092-8674(87)90220-0 [doi]
PST - ppublish
SO  - Cell. 1987 Jul 17;50(2):247-58. doi: 10.1016/0092-8674(87)90220-0.