PMID- 35948810
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20220926
IS  - 1568-5608 (Electronic)
IS  - 0925-4692 (Linking)
VI  - 30
IP  - 5
DP  - 2022 Oct
TI  - Tumor necrosis factor alpha (TNF-alpha) blockage reduces acute inflammation and 
      delayed wound healing in oral ulcer of rats.
PG  - 1781-1798
LID - 10.1007/s10787-022-01046-3 [doi]
AB  - Oral traumatic ulcers (OTU) are common in dental routine, and the control of 
      proinflammatory cytokines, such as the tumor necrosis factor-alpha (TNF-alpha), may 
      interfere with OTU repair. Our aim was to evaluate the role of TNF-alpha in the 
      healing process of OTU in rats. Wistar male rats were divided into six groups: a 
      control-group (treated with 0.1 mL/kg of saline) and five groups treated with 
      anti-TNF-alpha infliximab (INF) at 1, 3, 5, 7, and 10 mg/kg immediately before OTU 
      production. The animals were weighed (day 0) and euthanized on days 1, 3, 7, 14 
      and 21 after ulceration. The ulcers were clinically measured, and the mucosa 
      samples were histologically (scores 0-4), histochemically (collagen assay 
      (pircrosirius)), histomorphometrically (cell counting), and immunohistochemically 
      (TNF-alpha, alpha-smooth-muscle-actin (alpha-SMA), monocyte-chemoattractive-protein-1 
      (MCP-1), interleukin-8 (IL-8), and fibroblast-growth-factor (FGF)) analyzed. The 
      Evans blue assay was used to measure the vascular permeability. 
      ANOVA-1-2-way/Bonferroni, Kruskal-Wallis/Dunn, and correlation analyses were 
      performed (GraphPad Prism 5.0, p < 0.05). High doses of INF reduced the OTU area 
      (p = 0.043), body mass loss (p = 0.023), vascular permeability (p < 0.001), and 
      reduced delayed histologic scores (p < 0.05), polymorphonuclear (p < 0.001) and 
      mononuclear (p < 0.001) cells, blood vessel counting (p = 0.006), and total 
      (p < 0.001), type-I (p = 0.018), and type-III (p < 0.001) collagen. INF treatment 
      reduced TNF-alpha immunostaining and delayed MPC-1, FGF, and alpha-SMA expression, with 
      little/none influence in IL-8 immunostaining. TNF-alpha blockage by INF reduced acute 
      inflammation in OTU but delayed cell migration and wound healing.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
FAU - Freitas, Milena Oliveira
AU  - Freitas MO
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil.
AD  - Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, 
      Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil.
FAU - Fonseca, Alline Pereira Rodrigues
AU  - Fonseca APR
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil.
FAU - de Aguiar, Maria Thaynara
AU  - de Aguiar MT
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil.
FAU - Dias, Camila Costa
AU  - Dias CC
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil.
FAU - Avelar, Rafael Linard
AU  - Avelar RL
AD  - Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, 
      Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil.
FAU - Sousa, Fabricio Bitu
AU  - Sousa FB
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil.
AD  - Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, 
      Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil.
FAU - Alves, Ana Paula Negreiros Nunes
AU  - Alves APNN
AD  - Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, 
      Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil.
FAU - de Barros Silva, Paulo Goberlanio
AU  - de Barros Silva PG
AUID- ORCID: 0000-0002-1513-9027
AD  - Department of Dentistry, Unichristus, Rua Joao Adolfo Gurgel, 133, Coco, 
      Fortaleza, Ceara, CEP 60192-345, Brazil. paulo_goberlanio@yahoo.com.br.
AD  - Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, 
      Dentistry and Nursing, Federal University of Ceara, Fortaleza, Ceara, Brazil. 
      paulo_goberlanio@yahoo.com.br.
LA  - eng
PT  - Journal Article
DEP - 20220810
PL  - Switzerland
TA  - Inflammopharmacology
JT  - Inflammopharmacology
JID - 9112626
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 45PG892GO1 (Evans Blue)
RN  - 9007-34-5 (Collagen)
RN  - B72HH48FLU (Infliximab)
SB  - IM
MH  - Actins
MH  - Animals
MH  - Collagen
MH  - Cytokines
MH  - Evans Blue/therapeutic use
MH  - Inflammation/drug therapy
MH  - Infliximab/pharmacology/therapeutic use
MH  - Interleukin-8/therapeutic use
MH  - Male
MH  - *Oral Ulcer/drug therapy/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor Inhibitors
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Ulcer
MH  - Wound Healing
OTO - NOTNLM
OT  - Inflammation
OT  - Infliximab
OT  - Oral ulcer
OT  - Tumor necrosis factor tumoral-alpha
OT  - Wound healing
EDAT- 2022/08/11 06:00
MHDA- 2022/09/28 06:00
CRDT- 2022/08/10 23:30
PHST- 2022/06/20 00:00 [received]
PHST- 2022/07/26 00:00 [accepted]
PHST- 2022/08/11 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/08/10 23:30 [entrez]
AID - 10.1007/s10787-022-01046-3 [pii]
AID - 10.1007/s10787-022-01046-3 [doi]
PST - ppublish
SO  - Inflammopharmacology. 2022 Oct;30(5):1781-1798. doi: 10.1007/s10787-022-01046-3. 
      Epub 2022 Aug 10.