PMID- 35949154
OWN - NLM
STAT- MEDLINE
DCOM- 20220907
LR  - 20230811
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 34
IP  - 5
DP  - 2022 Sep
TI  - Considerations in the use of processing fluids for the detection of PRRSV RNA and 
      antibody.
PG  - 859-863
LID - 10.1177/10406387221114855 [doi]
AB  - Surveillance is mandatory for tracking the progress of porcine reproductive and 
      respiratory syndrome virus (PRRSV) control and elimination efforts in breeding 
      herds. Processing fluids, the fluid recovered from tissues collected at 
      castration and/or tail docking, are used for breeding herd surveillance by large 
      segments of the industry, but the basic diagnostic characteristics of processing 
      fluids are largely undescribed. We undertook 3 studies to address this 
      information gap. In study 1, we found no differences among the PRRSV RT-rtPCR 
      results obtained with 4 commercial RNA extraction kits. In study 2, we found that 
      PRRSV RNA was highly stable in processing fluid samples at -20 degrees C or 4 degrees C, but 
      detrimental effects were observed at >/=22 degrees C within 24 h. In study 3, using a 
      modified PRRSV ELISA at a sample:positive cutoff of >/=0.5, we found excellent 
      discrimination in the detection of PRRSV antibody (IgM, IgA, IgG) in processing 
      fluids from herds of known PRRSV status. Judicious handling of processing fluid 
      samples from sow herds, and the use of methods available in veterinary diagnostic 
      laboratories, can provide a foundation for reliable PRRSV surveillance.
FAU - Lopez, Will
AU  - Lopez W
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA; PIC North America, 
      Hendersonville, TN, USA.
FAU - Zimmerman, Jeff
AU  - Zimmerman J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Gauger, Phil
AU  - Gauger P
AUID- ORCID: 0000-0003-2540-8769
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Harmon, Karen
AU  - Harmon K
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Magtoto, Ronaldo
AU  - Magtoto R
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Bradner, Laura
AU  - Bradner L
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Holtkamp, Derald
AU  - Holtkamp D
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Statistics, College of Liberal Arts and Sciences.
FAU - Zhang, Jianqiang
AU  - Zhang J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Ramirez, Alejandro
AU  - Ramirez A
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Linhares, Daniel
AU  - Linhares D
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
FAU - Gimenez-Lirola, Luis
AU  - Gimenez-Lirola L
AUID- ORCID: 0000-0002-4407-7996
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine.
LA  - eng
PT  - Journal Article
DEP - 20220810
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Antibodies, Viral)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay/methods/veterinary
MH  - Female
MH  - *Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - RNA
MH  - Saliva
MH  - Swine
MH  - *Swine Diseases
PMC - PMC9446294
OTO - NOTNLM
OT  - ELISA
OT  - PCR
OT  - PRRSV
OT  - PRRSV RNA
OT  - antibody
OT  - pigs
OT  - processing fluids
OT  - surveillance
COIS- Declaration of conflicting interests: The authors declare no conflicts of 
      interest with respect to the research, authorship, and/or the publication of this 
      manuscript, with the exception that Jeff Zimmerman serves as a consultant to 
      Idexx Laboratories. The terms of the consulting arrangement have been reviewed 
      and approved by Iowa State University in accordance with its conflict-of-interest 
      policies.
EDAT- 2022/08/12 06:00
MHDA- 2022/09/08 06:00
PMCR- 2023/08/10
CRDT- 2022/08/11 01:33
PHST- 2022/08/12 06:00 [pubmed]
PHST- 2022/09/08 06:00 [medline]
PHST- 2022/08/11 01:33 [entrez]
PHST- 2023/08/10 00:00 [pmc-release]
AID - 10.1177_10406387221114855 [pii]
AID - 10.1177/10406387221114855 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2022 Sep;34(5):859-863. doi: 10.1177/10406387221114855. Epub 
      2022 Aug 10.