PMID- 35950376
OWN - NLM
STAT- MEDLINE
DCOM- 20220826
LR  - 20230118
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 10
IP  - 33
DP  - 2022 Aug 24
TI  - Exploration of biomimetic poly(gamma-benzyl-L-glutamate) fibrous scaffolds for 
      corneal nerve regeneration.
PG  - 6372-6379
LID - 10.1039/d2tb01250b [doi]
AB  - Poly(gamma-benzyl-L-glutamate) (PBG) made biomimetic scaffolds are explored as 
      candidate materials for corneal nerve regeneration and neurotrophic keratopathy 
      treatment. The PBG with built-in neurotransmitter glutamate was synthesized and 
      fabricated into 3D fibrous scaffolds containing aligned fibers using 
      electrospinning. In in vitro experiments, primary mouse trigeminal ganglia (TG) 
      cells were used. Immunohistochemistry (IHC) analysis shows that TG cells cultured 
      on PBG have no cytotoxic response for 21 days. Without any nerve growth factor, 
      TG cells have the longest neurite length of 225.3 mum in the PBG group and 1.3 
      times the average length as compared with the polycaprolactone and no scaffold 
      groups. Also, aligned fibers guide the neurite growth and extension 
      unidirectionally. In vivo assays were carried out by intracorneal implantation of 
      PBG on clinical New Zealand rabbits. The external eye photos and in vivo confocal 
      microscopy (IVCM) show a low immune response. The corneal neural markers (betaIII 
      tubulin and SMI312) in the IHC analysis are consistent with the position stained 
      by glutamate of implanted scaffolds, indicating that PBG induces neurogenesis. 
      PBG exhibits mechanical stiffness to resist material deformation possibly caused 
      by surgical operations. The results of this study demonstrate that PBG is 
      suitable for corneal nerve regeneration and the treatment of neurotrophic 
      keratopathy.
FAU - Ma, Tien-Li
AU  - Ma TL
AUID- ORCID: 0000-0002-6241-9961
AD  - Department of Materials Science and Engineering, National Taiwan University, 
      Taipei, Taiwan. suwf@ntu.edu.tw.
FAU - Yang, Shang-Chih
AU  - Yang SC
AD  - Department of Ophthalmology, National Taiwan University College of Medicine, 
      Taipei, Taiwan. chenweili@ntu.edu.tw.
FAU - Cheng, Ting
AU  - Cheng T
AD  - Department of Ophthalmology, National Taiwan University College of Medicine, 
      Taipei, Taiwan. chenweili@ntu.edu.tw.
FAU - Chen, Mei-Yun
AU  - Chen MY
AD  - Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
FAU - Wu, Jo-Hsuan
AU  - Wu JH
AD  - Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University 
      of California, San Diego, California, USA.
FAU - Liao, Shu-Lang
AU  - Liao SL
AD  - Department of Ophthalmology, National Taiwan University College of Medicine, 
      Taipei, Taiwan. chenweili@ntu.edu.tw.
AD  - Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
FAU - Chen, Wei-Li
AU  - Chen WL
AD  - Department of Ophthalmology, National Taiwan University College of Medicine, 
      Taipei, Taiwan. chenweili@ntu.edu.tw.
AD  - Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
AD  - Advanced Ocular Surface and Corneal Nerve Regeneration Center, National Taiwan 
      University Hospital, Taipei, Taiwan.
FAU - Su, Wei-Fang
AU  - Su WF
AUID- ORCID: 0000-0002-3375-4664
AD  - Department of Materials Science and Engineering, National Taiwan University, 
      Taipei, Taiwan. suwf@ntu.edu.tw.
AD  - Department of Materials Engineering, Ming Chi University of Technology, New 
      Taipei City, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220824
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
EIN - J Mater Chem B. 2023 Feb 1;11(5):1159. PMID: 36651517
MH  - Animals
MH  - Biomimetics
MH  - *Glutamic Acid
MH  - Mice
MH  - Nerve Regeneration/physiology
MH  - Neurites
MH  - Rabbits
MH  - *Tissue Scaffolds
EDAT- 2022/08/12 06:00
MHDA- 2022/08/27 06:00
CRDT- 2022/08/11 04:13
PHST- 2022/08/12 06:00 [pubmed]
PHST- 2022/08/27 06:00 [medline]
PHST- 2022/08/11 04:13 [entrez]
AID - 10.1039/d2tb01250b [doi]
PST - epublish
SO  - J Mater Chem B. 2022 Aug 24;10(33):6372-6379. doi: 10.1039/d2tb01250b.