PMID- 35953439
OWN - NLM
STAT- MEDLINE
DCOM- 20221216
LR  - 20221221
IS  - 1808-8686 (Electronic)
IS  - 1808-8694 (Print)
IS  - 1808-8686 (Linking)
VI  - 88 Suppl 4
IP  - Suppl 4
DP  - 2022 Nov-Dec
TI  - MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting 
      CST1.
PG  - S108-S116
LID - S1808-8694(22)00097-0 [pii]
LID - 10.1016/j.bjorl.2022.06.005 [doi]
AB  - OBJECTIVE: This study aims to explore the effect and mechanism of miR-375 in 
      Laryngeal Squamous Cell Carcinoma (LSCC) cell progression. METHODS: LSCC cells 
      (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or 
      miR-375-mimic+oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was 
      measured. The effect of miR-375-mimic, miR-375-inhibitor or miR-375-mimic+oe-CST1 
      on cell biological functions, including cell proliferation, migration, invasion, 
      and apoptosis, was also assessed. The potential relationship between CST1 and 
      miR-375 was predicted by Jefferson software and validated by dual luciferase 
      reporter gene assay. RESULTS: Downregulated miR-375 expression was found in LSCC 
      cells. Overexpression of miR-375 inhibited the viability and migration and 
      promoted apoptosis of LSCC cells. Jefferson database and dual luciferase reporter 
      gene assay confirmed that miR-375 directly targeted CST1. Overexpression of CST1 
      could reverse the anti-cancer effect of miR-375 overexpression in LSCC cells. 
      CONCLUSION: Collected evidence showed that miR-375/CST1 axis was implicated in 
      LSCC progression. LEVEL OF EVIDENCE: Level 3.
CI  - Copyright (c) 2022 Associacao Brasileira de Otorrinolaringologia e Cirurgia 
      Cervico-Facial. Published by Elsevier Editora Ltda. All rights reserved.
FAU - Dai, Feng
AU  - Dai F
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China. Electronic address: daifeng3210@163.com.
FAU - Xie, Zuojun
AU  - Xie Z
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China.
FAU - Yang, Qiming
AU  - Yang Q
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China.
FAU - Zhong, Zhuanglong
AU  - Zhong Z
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China.
FAU - Zhong, Chun
AU  - Zhong C
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China.
FAU - Qiu, Yongliang
AU  - Qiu Y
AD  - Jiangxi Pingxiang People's Hospital, Department of Otorhinolaryngology Head and 
      Neck Surgery, Pingxiang, China.
LA  - eng
PT  - Journal Article
DEP - 20220706
PL  - Brazil
TA  - Braz J Otorhinolaryngol
JT  - Brazilian journal of otorhinolaryngology
JID - 101207337
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN375 microRNA, human)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - *MicroRNAs/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Head and Neck Neoplasms
PMC - PMC9756075
OTO - NOTNLM
OT  - Cystatin SN
OT  - Invasion
OT  - Laryngeal squamous cell carcinoma
OT  - Migration
OT  - Proliferation
OT  - miR-375
EDAT- 2022/08/12 06:00
MHDA- 2022/12/15 06:00
PMCR- 2022/07/06
CRDT- 2022/08/11 22:09
PHST- 2022/02/06 00:00 [received]
PHST- 2022/04/20 00:00 [revised]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/08/12 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
PHST- 2022/08/11 22:09 [entrez]
PHST- 2022/07/06 00:00 [pmc-release]
AID - S1808-8694(22)00097-0 [pii]
AID - 10.1016/j.bjorl.2022.06.005 [doi]
PST - ppublish
SO  - Braz J Otorhinolaryngol. 2022 Nov-Dec;88 Suppl 4(Suppl 4):S108-S116. doi: 
      10.1016/j.bjorl.2022.06.005. Epub 2022 Jul 6.