PMID- 35955675
OWN - NLM
STAT- MEDLINE
DCOM- 20220815
LR  - 20220815
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 15
DP  - 2022 Aug 1
TI  - Psychological Stress Exacerbates Inflammation of the Ileum via the 
      Corticotropin-Releasing Hormone-Mast Cell Axis in a Mouse Model of Eosinophilic 
      Enteritis.
LID - 10.3390/ijms23158538 [doi]
LID - 8538
AB  - The effects of psychological stress on eosinophilic gastrointestinal disorders 
      have not been elucidated. This study investigated the effects of psychological 
      stress in a mouse model of eosinophilic enteritis (EoN). BALB/c mice were treated 
      with ovalbumin (OVA) to create an EoN model and subjected to either water 
      avoidance stress (WAS) or sham stress (SS). Microscopic inflammation, eosinophil 
      and mast cell counts, mRNA expression, and protein levels of type 2 helper T cell 
      (Th2) cytokines in the ileum were compared between groups. We evaluated ex vivo 
      intestinal permeability using an Ussing chamber. A corticotropin-releasing 
      hormone type 1 receptor (CRH-R1) antagonist was administered before WAS, and its 
      effects were analyzed. WAS significantly increased diarrhea occurrence and, 
      eosinophil and mast cell counts, and decreased the villus/crypt ratio compared to 
      those in the SS group. The mRNA expression of CRH, interleukin IL-4, IL-5, IL-13, 
      eotaxin-1, and mast cell tryptase beta2 significantly increased, and the protein 
      levels of IL-5, IL-13, and OVA-specific immunoglobulin E (IgE) also significantly 
      increased in the WAS group. Moreover, WAS significantly increased the intestinal 
      permeability. The CRH-R1 antagonist significantly inhibited all changes induced 
      by WAS. Psychological stress exacerbated ileal inflammation via the CRH-mast cell 
      axis in an EoN mouse model.
FAU - Kanamori, Atsushi
AU  - Kanamori A
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka City 
      University, Osaka 545-8585, Japan.
FAU - Tanaka, Fumio
AU  - Tanaka F
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Ominami, Masaki
AU  - Ominami M
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Nadatani, Yuji
AU  - Nadatani Y
AUID- ORCID: 0000-0001-7081-8746
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
AD  - Department of Premier Preventive Medicine/MedCity21, Graduate School of Medicine, 
      Osaka Metropolitan University, Osaka 545-8585, Japan.
FAU - Fukunaga, Shusei
AU  - Fukunaga S
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Otani, Koji
AU  - Otani K
AUID- ORCID: 0000-0001-9075-6415
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Hosomi, Shuhei
AU  - Hosomi S
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Kamata, Noriko
AU  - Kamata N
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Nagami, Yasuaki
AU  - Nagami Y
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Taira, Koichi
AU  - Taira K
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
FAU - Fujiwara, Yasuhiro
AU  - Fujiwara Y
AD  - Department of Gastroenterology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka 545-8585, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220801
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - Eosinophilic enteropathy
SB  - IM
MH  - Animals
MH  - *Corticotropin-Releasing Hormone/metabolism
MH  - Disease Models, Animal
MH  - Enteritis
MH  - Eosinophilia
MH  - Gastritis
MH  - Ileum/metabolism
MH  - Inflammation/metabolism
MH  - Interleukin-13/metabolism
MH  - Interleukin-5
MH  - *Mast Cells/metabolism
MH  - Mice
MH  - RNA, Messenger/metabolism
MH  - Receptors, Corticotropin-Releasing Hormone/genetics/metabolism
MH  - Stress, Psychological/complications
PMC - PMC9369025
OTO - NOTNLM
OT  - corticotropin-releasing hormone
OT  - eosinophilic enteritis
OT  - eosinophilic gastrointestinal disorders
OT  - eosinophils
OT  - intestinal permeability
OT  - mast cells
OT  - psychological stress
COIS- Y.F. had grant support from DAIICHI SANKYO COMPANY, LIMITED (Tokyo, Japan) and EA 
      Pharma Co., Ltd (Tokyo, Japan).
EDAT- 2022/08/13 06:00
MHDA- 2022/08/16 06:00
PMCR- 2022/08/01
CRDT- 2022/08/12 01:12
PHST- 2022/07/07 00:00 [received]
PHST- 2022/07/26 00:00 [revised]
PHST- 2022/07/29 00:00 [accepted]
PHST- 2022/08/12 01:12 [entrez]
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/08/16 06:00 [medline]
PHST- 2022/08/01 00:00 [pmc-release]
AID - ijms23158538 [pii]
AID - ijms-23-08538 [pii]
AID - 10.3390/ijms23158538 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Aug 1;23(15):8538. doi: 10.3390/ijms23158538.