PMID- 35955677
OWN - NLM
STAT- MEDLINE
DCOM- 20220815
LR  - 20220901
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 15
DP  - 2022 Aug 1
TI  - Detailed Characterization of Small Extracellular Vesicles from Different Cell 
      Types Based on Tetraspanin Composition by ExoView R100 Platform.
LID - 10.3390/ijms23158544 [doi]
LID - 8544
AB  - Small extracellular vesicles (sEV) hold enormous potential as biomarkers, drug 
      carriers, and therapeutic agents. However, due to previous limitations in the 
      phenotypic characterization of sEV at the single vesicle level, knowledge of cell 
      type-specific sEV signatures remains sparse. With the introduction of 
      next-generation sEV analysis devices, such as the single-particle interferometric 
      reflectance imaging sensor (SP-IRIS)-based ExoView R100 platform, single sEV 
      analyses are now possible. While the tetraspanins CD9, CD63, and CD81 were 
      generally considered pan-sEV markers, it became clear that sEV of different cell 
      types contain several combinations and amounts of these proteins on their 
      surfaces. To gain better insight into the complexity and heterogeneity of sEV, we 
      used the ExoView R100 platform to analyze the CD9/CD63/CD81 phenotype of sEV 
      released by different cell types at a single sEV level. We demonstrated that 
      these surface markers are sufficient to distinguish cell-type-specific sEV 
      phenotypes. Furthermore, we recognized that tetraspanin composition in some sEV 
      populations does not follow a random pattern. Notably, the tetraspanin 
      distribution of sEV derived from mesenchymal stem cells (MSCs) alters depending 
      on cell culture conditions. Overall, our data provide an overview of the 
      cell-specific characteristics of sEV populations, which will increase the 
      understanding of sEV physiology and improve the development of new sEV-based 
      therapeutic approaches.
FAU - Breitwieser, Kai
AU  - Breitwieser K
AD  - Fachbereich Biologie, Technische Universitat Darmstadt, 64287 Darmstadt, Germany.
FAU - Koch, Leon F
AU  - Koch LF
AD  - Fachbereich Biologie, Technische Universitat Darmstadt, 64287 Darmstadt, Germany.
FAU - Tertel, Tobias
AU  - Tertel T
AD  - Institute for Transfusion Medicine, University Hospital Essen, University of 
      Duisburg-Essen, 45122 Essen, Germany.
FAU - Proestler, Eva
AU  - Proestler E
AD  - Fachbereich Biologie, Technische Universitat Darmstadt, 64287 Darmstadt, Germany.
FAU - Burgers, Luisa D
AU  - Burgers LD
AD  - Institute of Pharmaceutical Biology, Goethe University Frankfurt, 60438 Frankfurt 
      am Main, Germany.
FAU - Lipps, Christoph
AU  - Lipps C
AUID- ORCID: 0000-0002-9350-5854
AD  - Department of Internal Medicine I, Cardiology, Justus-Liebig-University Giessen, 
      35392 Giessen, Germany.
FAU - Adjaye, James
AU  - Adjaye J
AUID- ORCID: 0000-0002-6075-6761
AD  - Institute for Stem Cell Research and Regenerative Medicine, Universitatsklinikum 
      Dusseldorf, 40225 Dusseldorf, Germany.
FAU - Furst, Robert
AU  - Furst R
AUID- ORCID: 0000-0002-9926-7578
AD  - Institute of Pharmaceutical Biology, Goethe University Frankfurt, 60438 Frankfurt 
      am Main, Germany.
FAU - Giebel, Bernd
AU  - Giebel B
AUID- ORCID: 0000-0003-2446-948X
AD  - Institute for Transfusion Medicine, University Hospital Essen, University of 
      Duisburg-Essen, 45122 Essen, Germany.
FAU - Saul, Meike J
AU  - Saul MJ
AD  - Fachbereich Biologie, Technische Universitat Darmstadt, 64287 Darmstadt, Germany.
LA  - eng
GR  - Dr. Hans Messer-Foundation Award 2021/Dr. Hans Messer-Foundation/
GR  - 2018.115.1/Wilhelm Sander-Foundation/
GR  - Athene Young Investigator program/Technische Universitat Darmstadt/
GR  - n/a/Dr. Ing. Wilhelm und Maria Kirmser Foundation/
PT  - Journal Article
DEP - 20220801
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (Tetraspanin 30)
RN  - 0 (Tetraspanins)
SB  - IM
MH  - Biomarkers/metabolism
MH  - *Extracellular Vesicles/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Tetraspanin 30/metabolism
MH  - Tetraspanins/metabolism
PMC - PMC9369185
OTO - NOTNLM
OT  - characterization
OT  - phenotype
OT  - small extracellular vesicles
OT  - tetraspanins
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/13 06:00
MHDA- 2022/08/16 06:00
PMCR- 2022/08/01
CRDT- 2022/08/12 01:12
PHST- 2022/06/27 00:00 [received]
PHST- 2022/07/21 00:00 [revised]
PHST- 2022/07/25 00:00 [accepted]
PHST- 2022/08/12 01:12 [entrez]
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/08/16 06:00 [medline]
PHST- 2022/08/01 00:00 [pmc-release]
AID - ijms23158544 [pii]
AID - ijms-23-08544 [pii]
AID - 10.3390/ijms23158544 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Aug 1;23(15):8544. doi: 10.3390/ijms23158544.