PMID- 35955914
OWN - NLM
STAT- MEDLINE
DCOM- 20220815
LR  - 20220831
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 15
DP  - 2022 Aug 7
TI  - Characterization of the Basal and mTOR-Dependent Acute Pulmonary and Systemic 
      Immune Response in a Murine Model of Combined Burn and Inhalation Injury.
LID - 10.3390/ijms23158779 [doi]
LID - 8779
AB  - Severe burn injury leads to a cascade of local and systemic immune responses that 
      trigger an extreme state of immune dysfunction, leaving the patient highly 
      susceptible to acute and chronic infection. When combined with inhalation injury, 
      burn patients have higher mortality and a greater chance of developing secondary 
      respiratory complications including infection. No animal model of combined burn 
      and inhalation injury (B+I) exists that accurately mirrors the human clinical 
      picture, nor are there any effective immunotherapies or predictive models of the 
      risk of immune dysfunction. Our earlier work showed that the 
      mechanistic/mammalian target of rapamycin (mTOR) pathway is activated early after 
      burn injury, and its chemical blockade at injury reduced subsequent chronic 
      bacterial susceptibility. It is unclear if mTOR plays a role in the exacerbated 
      immune dysfunction seen after B+I injury. We aimed to: (1) characterize a novel 
      murine model of B+I injury, and (2) investigate the role of mTOR in the immune 
      response after B+I injury. Pulmonary and systemic immune responses to B+I were 
      characterized in the absence or presence of mTOR inhibition at the time of 
      injury. Data describe a murine model of B+I with inhalation-specific immune 
      phenotypes and implicate mTOR in the acute immune dysfunction observed.
FAU - Hall, Hannah R
AU  - Hall HR
AD  - Department of Pathology and Laboratory Medicine, University of North Carolina at 
      Chapel Hill, Chapel Hill, NC 27599, USA.
AD  - North Carolina Jaycee Burn Center, Department of Surgery, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
FAU - Mahung, Cressida
AU  - Mahung C
AD  - North Carolina Jaycee Burn Center, Department of Surgery, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
FAU - Dunn, Julia L M
AU  - Dunn JLM
AD  - Department of Microbiology and Immunology, University of North Carolina at Chapel 
      Hill, Chapel Hill, NC 27599, USA.
FAU - Kartchner, Laurel M
AU  - Kartchner LM
AD  - Department of Microbiology and Immunology, University of North Carolina at Chapel 
      Hill, Chapel Hill, NC 27599, USA.
FAU - Seim, Roland F
AU  - Seim RF
AUID- ORCID: 0000-0001-9023-4502
AD  - North Carolina Jaycee Burn Center, Department of Surgery, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
AD  - Curriculum in Toxicology and Environmental Medicine, University of North Carolina 
      at Chapel Hill, Chapel Hill, NC 27599, USA.
FAU - Cairns, Bruce A
AU  - Cairns BA
AD  - North Carolina Jaycee Burn Center, Department of Surgery, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
AD  - Department of Microbiology and Immunology, University of North Carolina at Chapel 
      Hill, Chapel Hill, NC 27599, USA.
FAU - Wallet, Shannon M
AU  - Wallet SM
AD  - Department of Microbiology and Immunology, University of North Carolina at Chapel 
      Hill, Chapel Hill, NC 27599, USA.
AD  - Division of Oral and Craniofacial Health Sciences, University of North Carolina 
      Adams School of Dentistry, Chapel Hill, NC 27599, USA.
FAU - Maile, Robert
AU  - Maile R
AUID- ORCID: 0000-0002-9126-4188
AD  - North Carolina Jaycee Burn Center, Department of Surgery, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
AD  - Department of Microbiology and Immunology, University of North Carolina at Chapel 
      Hill, Chapel Hill, NC 27599, USA.
AD  - Curriculum in Toxicology and Environmental Medicine, University of North Carolina 
      at Chapel Hill, Chapel Hill, NC 27599, USA.
LA  - eng
GR  - R01 GM131124/GM/NIGMS NIH HHS/United States
GR  - R01GM131124/GM/NIGMS NIH HHS/United States
GR  - T32ES007126/ES/NIEHS NIH HHS/United States
GR  - 5T32GM008450/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20220807
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Burns/metabolism
MH  - Disease Models, Animal
MH  - Humans
MH  - Immunity
MH  - Immunotherapy
MH  - *Lung Injury/complications
MH  - Mammals
MH  - Mice
MH  - TOR Serine-Threonine Kinases
PMC - PMC9368856
OTO - NOTNLM
OT  - burn injury
OT  - inhalation injury
OT  - mTOR
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/13 06:00
MHDA- 2022/08/16 06:00
PMCR- 2022/08/07
CRDT- 2022/08/12 01:14
PHST- 2022/07/01 00:00 [received]
PHST- 2022/07/26 00:00 [revised]
PHST- 2022/08/04 00:00 [accepted]
PHST- 2022/08/12 01:14 [entrez]
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/08/16 06:00 [medline]
PHST- 2022/08/07 00:00 [pmc-release]
AID - ijms23158779 [pii]
AID - ijms-23-08779 [pii]
AID - 10.3390/ijms23158779 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Aug 7;23(15):8779. doi: 10.3390/ijms23158779.