PMID- 35957817
OWN - NLM
STAT- MEDLINE
DCOM- 20220815
LR  - 20220829
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - 17beta-Estradiol (E2) may be involved in the mode of crustacean female sex hormone 
      (CFSH) action in the blue crab, Callinectes sapidus.
PG  - 962576
LID - 10.3389/fendo.2022.962576 [doi]
LID - 962576
AB  - 17beta-estradiol (E2) has been proved to control reproduction, sexual 
      differentiation, and the development of the secondary sexual characteristics of 
      vertebrate females. In decapod crustacean species, crustacean female sex hormone 
      (CFSH), a protein hormone, is required for developing adult-specific ovigerous 
      setae for embryo brooding and gonophores for mating at the blue crab Callinectes 
      sapidus puberty molting. However, it is unclear that whether the mode of CFSH 
      action involves a vertebrate-type sex steroid hormone in crustaceans. To this 
      end, E2 levels were first measured using a competitive ELISA in the hemolymph and 
      the potential CFSH target tissues from both prepuberty and adult females; the 
      presence of E2 was further confirmed with a liquid chromatography tandem mass 
      spectrometry method. Then, the cDNAs of the following genes known to be 
      associated with vertebrate steroidogenic pathways were isolated: StAR-related 
      lipid transfer protein 3 (StAR3); 3beta-hydroxysteroid dehydrogenase (3betaHSD); two 
      isoforms of 17beta-hydroxysteroid dehydrogenase 8 (17betaHSD8); and, estradiol-related 
      receptor (ERR). RT-PCR analysis revealed that these genes were widely distributed 
      in the eyestalk ganglia, hepatopancreas, brain, ovary, spermathecae, ovigerous 
      and plumose setae tissues of adult females. The 17betaHSD8 transcripts were 
      localized in the follicle cells, the periphery of the nuclear membrane of primary 
      oocytes, and yolk granules of the vitellogenic oocytes using in situ 
      hybridization, and the corresponding protein was detected in the follicle cells 
      and ooplasm of primary oocytes using immunohistochemistry. Furthermore, the adult 
      females injected with CFSH-dsRNA (n = 30 times) had E2 and StAR3 transcripts 
      levels lower in the ovigerous and plumose setae, spermathecae than controls. 
      These results suggested that the mode of CFSH action in C. sapidus might involve 
      E2 in these adult-female-specific tissues.
CI  - Copyright (c) 2022 Wang, He, Blaney and Chung.
FAU - Wang, Tao
AU  - Wang T
AD  - Department of Marine Biotechnology & Institute of Marine and Environmental 
      Technology, University of Maryland Baltimore County, Baltimore, MD, United 
      States.
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science, Baltimore, MD, United States.
FAU - He, Ke
AU  - He K
AD  - Department of Chemical, Biochemical, and Environmental Engineering, University of 
      Maryland Baltimore County, Baltimore, MD, United States.
FAU - Blaney, Lee
AU  - Blaney L
AD  - Department of Chemical, Biochemical, and Environmental Engineering, University of 
      Maryland Baltimore County, Baltimore, MD, United States.
FAU - Chung, J Sook
AU  - Chung JS
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science, Baltimore, MD, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20220725
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - 0 (Gonadal Steroid Hormones)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - *Brachyura/genetics
MH  - Estradiol
MH  - Female
MH  - Gonadal Steroid Hormones/metabolism
MH  - Hepatopancreas/metabolism
MH  - Sexual Maturation
PMC - PMC9358259
OTO - NOTNLM
OT  - 17beta-estradiol (E2)
OT  - Callinectes sapidus
OT  - crustacean female sex hormone (CFSH)
OT  - sexual differentiation
OT  - steroidogenesis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/13 06:00
MHDA- 2022/08/16 06:00
PMCR- 2022/01/01
CRDT- 2022/08/12 02:46
PHST- 2022/06/06 00:00 [received]
PHST- 2022/06/28 00:00 [accepted]
PHST- 2022/08/12 02:46 [entrez]
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/08/16 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.962576 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Jul 25;13:962576. doi: 
      10.3389/fendo.2022.962576. eCollection 2022.