PMID- 35958678
OWN - NLM
STAT- MEDLINE
DCOM- 20220815
LR  - 20220817
IS  - 1918-1523 (Electronic)
IS  - 1203-6765 (Print)
IS  - 1203-6765 (Linking)
VI  - 2022
DP  - 2022
TI  - CAV-1 Overexpression Exacerbates the Manifestation in EPAC-1-Induced Chronic 
      Postsurgical Pain in Rats.
PG  - 8566840
LID - 10.1155/2022/8566840 [doi]
LID - 8566840
AB  - PURPOSE: Caveolae (CAV) are an invaginated microcapsule with the shape of Omega on 
      the surface of the cell membrane. Caveolin-1 (CAV-1) is involved in neuropathic 
      pain, and adenosine monophosphate (AMP)-exchange protein directly activated by 
      cAMP1 (EPAC-1) is a potential therapeutic target for chronic pain. However, 
      whether EPAC-1 promotes chronic postsurgical pain (CPSP) through CAV-1 has not 
      been reported. Here, we aim to investigate the underlying mechanism of CAV in 
      CPSP. METHODS: All the rats were divided into 9 groups, including the Naive 
      group, Sham group, skin/muscle incision and retraction (SMIR) group, SMIR + CAV-1 
      siRNA group, SMIR + control siRNA group, SMIR (7 days)+Saline group, SMIR 
      (7 days)+CE3F4 group, 8-PCPT group, and Saline group. The CPSP rat model was 
      established after SMIR. A mechanical withdrawal threshold (MWT) was recorded to 
      evaluate the animal's behavior. Western blotting and immunofluorescent were 
      performed to detect the protein expression levels of EPAC-1 and P-CAV-1. RESULTS: 
      EPAC-1 and CAV-1 were both overexpressed after operation, particularly in 
      astrocytes, microglia, and neurons of spinal marrow (all P < 0.05). 
      Interestingly, CAV-1 siRNA can partly reverse the SMIR-induced hypersensitivity, 
      but there was no effect on EPAC-1. Besides, EPAC-1 blockage partly reversed the 
      SMIR-induced hypersensitivity and CAV-1 overexpression, and EPAC-1 activation 
      promoted CAV-1 overexpression and hypersensitivity in normal rats (all P < 0.05). 
      CONCLUSION: CAV-1 mediates the functional coupling of microglia, astrocytes, and 
      neurons, and thus EPAC-1/CAV-1 plays an important role in CPSP exacerbation.
CI  - Copyright (c) 2022 Qian Hua et al.
FAU - Hua, Qian
AU  - Hua Q
AUID- ORCID: 0000-0002-3474-1777
AD  - Medical College, Nantong University, Nantong, Jiangsu 226001, China.
FAU - Shen, Shiren
AU  - Shen S
AUID- ORCID: 0000-0003-3547-5375
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu 226001, China.
FAU - Qin, Yibin
AU  - Qin Y
AUID- ORCID: 0000-0002-8484-0968
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu 226001, China.
FAU - Cao, Su
AU  - Cao S
AUID- ORCID: 0000-0003-0754-7087
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 
      University, Nantong, Jiangsu 226001, China.
LA  - eng
PT  - Journal Article
DEP - 20220731
PL  - United States
TA  - Pain Res Manag
JT  - Pain research & management
JID - 9612504
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - *Caveolae/metabolism
MH  - *Chronic Pain/etiology/metabolism
MH  - Guanine Nucleotide Exchange Factors/genetics
MH  - Pain, Postoperative/metabolism
MH  - RNA, Small Interfering
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC9357801
COIS- The authors declare no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2022/08/13 06:00
MHDA- 2022/08/16 06:00
PMCR- 2022/07/31
CRDT- 2022/08/12 03:01
PHST- 2021/07/28 00:00 [received]
PHST- 2022/01/06 00:00 [revised]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/08/12 03:01 [entrez]
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/08/16 06:00 [medline]
PHST- 2022/07/31 00:00 [pmc-release]
AID - 10.1155/2022/8566840 [doi]
PST - epublish
SO  - Pain Res Manag. 2022 Jul 31;2022:8566840. doi: 10.1155/2022/8566840. eCollection 
      2022.