PMID- 35960616
OWN - NLM
STAT- MEDLINE
DCOM- 20221205
LR  - 20230125
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2022
IP  - 12
DP  - 2022 Dec 1
TI  - Obtaining Polytene, Meiotic, and Mitotic Chromosomes from Mosquitoes for 
      Cytogenetic Analysis.
PG  - 591-598
LID - 10.1101/pdb.prot107872 [doi]
AB  - Chromosome visualization is a key step for developing cytogenetic maps and 
      idiograms, analyzing inversion polymorphisms, and identifying mosquito species. 
      Three types of chromosomes-polytene, mitotic, and meiotic-are used in cytogenetic 
      studies of mosquitoes. Here, we describe a detailed method for obtaining 
      high-quality polytene chromosome preparations from the salivary glands of larvae 
      and the ovaries of females for Anopheles mosquitoes. We also describe how to 
      obtain mitotic chromosomes from imaginal discs of fourth-instar larvae and 
      meiotic chromosomes from the testes of male pupae for all mosquitoes. These 
      chromosomes can be used for fluorescence in situ hybridization (FISH), a 
      fundamental technique in cytogenetic research that is used for physical genome 
      mapping, detecting chromosomal rearrangements, and studying chromosome 
      organization.
CI  - (c) 2022 Cold Spring Harbor Laboratory Press.
FAU - Liang, Jiangtao
AU  - Liang J
AD  - Department of Entomology, Virginia Polytechnic Institute and State University, 
      Blacksburg, Virginia 24060, USA.
FAU - Bondarenko, Simon M
AU  - Bondarenko SM
AD  - Department of Entomology, Virginia Polytechnic Institute and State University, 
      Blacksburg, Virginia 24060, USA.
AD  - Department of Genetics and Cell Biology, Tomsk State University, Tomsk 634050, 
      Russia.
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
AD  - Department of Entomology, Virginia Polytechnic Institute and State University, 
      Blacksburg, Virginia 24060, USA.
AD  - Department of Genetics and Cell Biology, Tomsk State University, Tomsk 634050, 
      Russia.
FAU - Sharakhova, Maria V
AU  - Sharakhova MV
AD  - Department of Entomology, Virginia Polytechnic Institute and State University, 
      Blacksburg, Virginia 24060, USA msharakh@vt.edu.
AD  - Laboratory of Evolutionary Genomics of Insects, Institute of Cytology and 
      Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, 
      Russia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221201
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
SB  - IM
MH  - Male
MH  - Animals
MH  - Female
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Polytene Chromosomes/genetics
MH  - Chromosomes/genetics
MH  - *Anopheles/genetics
MH  - Chromosome Mapping
MH  - Cytogenetic Analysis
MH  - Larva/genetics
EDAT- 2022/08/13 06:00
MHDA- 2022/12/06 06:00
CRDT- 2022/08/12 12:32
PHST- 2022/08/13 06:00 [pubmed]
PHST- 2022/12/06 06:00 [medline]
PHST- 2022/08/12 12:32 [entrez]
AID - pdb.prot107872 [pii]
AID - 10.1101/pdb.prot107872 [doi]
PST - epublish
SO  - Cold Spring Harb Protoc. 2022 Dec 1;2022(12):591-598. doi: 
      10.1101/pdb.prot107872.