PMID- 35963103
OWN - NLM
STAT- MEDLINE
DCOM- 20220920
LR  - 20220920
IS  - 1751-732X (Electronic)
IS  - 1751-7311 (Linking)
VI  - 16
IP  - 9
DP  - 2022 Sep
TI  - Glutathione S-transferase Mu 3 is associated to in vivo fertility, but not sperm 
      quality, in bovine.
PG  - 100609
LID - S1751-7311(22)00162-8 [pii]
LID - 10.1016/j.animal.2022.100609 [doi]
AB  - In the dairy breeding industry, pregnancy of dairy cows is essential to initiate 
      milk production, so that high fertility rates are required to increase their 
      productivity. In this regard, sperm proteins that are indicative of sperm quality 
      and/or fertility have become an important target of study. Glutathione 
      S-transferase Mu 3 (GSTM3) has been established as a fertility and sperm quality 
      parameter in humans and pigs and, consequently, it might be a potential biomarker 
      in cattle. For this reason, the present work aimed to determine if GSTM3 could 
      predict sperm quality and in vivo fertility in this species. Sperm quality was 
      assessed with flow cytometry and computer-assisted sperm analysis. Immunoblotting 
      and immunofluorescence analysis were performed to determine the presence and 
      localisation pattern of sperm GSTM3. This enzyme was found to be present in 
      bovine sperm and to be localised along the sperm tail and the equatorial segment 
      of the head. No significant associations between sperm GSTM3 and sperm quality 
      parameters were observed, except a negative association with morphologically 
      abnormal sperm having a coiled tail. In addition, and more relevant, higher 
      levels of GSTM3 in sperm were seen in bulls showing lower in vivo fertility 
      rates. In conclusion, our data evidenced the presence of GSTM3 in bovine sperm. 
      Moreover, we suggest that, despite not being associated with sperm quality, GSTM3 
      might be an in vivo subfertility biomarker in cattle sperm, and that high levels 
      of this protein could be an indicative of defective spermatogenesis and/or 
      epididymal maturation.
CI  - Copyright (c) 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Garriga, Ferran
AU  - Garriga F
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain.
FAU - Llavanera, Marc
AU  - Llavanera M
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain.
FAU - Vinolas-Verges, Estel
AU  - Vinolas-Verges E
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain.
FAU - Recuero, Sandra
AU  - Recuero S
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain.
FAU - Tamargo, Carolina
AU  - Tamargo C
AD  - Department of Animal Selection and Reproduction, The Regional Agri-Food Research 
      and Development Service of Asturias (SERIDA), ES-33394 Gijon, Spain.
FAU - Delgado-Bermudez, Ariadna
AU  - Delgado-Bermudez A
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain.
FAU - Yeste, Marc
AU  - Yeste M
AD  - Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food 
      and Agricultural Technology, University of Girona, ES-17003 Girona, Spain; Unit 
      of Cell Biology, Department of Biology, Faculty of Sciences, University of 
      Girona, ES-17003 Girona, Spain; Catalan Institution for Research and Advanced 
      Studies (ICREA), ES-08010 Barcelona, Spain. Electronic address: 
      marc.yeste@udg.edu.
LA  - eng
PT  - Journal Article
DEP - 20220811
PL  - England
TA  - Animal
JT  - Animal : an international journal of animal bioscience
JID - 101303270
RN  - 0 (Biomarkers)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cattle
MH  - Female
MH  - *Fertility
MH  - Glutathione Transferase/genetics/metabolism
MH  - Humans
MH  - Male
MH  - Semen Analysis/veterinary
MH  - Sperm Motility
MH  - *Spermatozoa/metabolism
MH  - Swine
OTO - NOTNLM
OT  - Biomarker
OT  - Dairy cattle
OT  - Flow cytometry
OT  - Non-return rate
OT  - Sperm quality
EDAT- 2022/08/14 06:00
MHDA- 2022/09/21 06:00
CRDT- 2022/08/13 18:20
PHST- 2022/01/17 00:00 [received]
PHST- 2022/07/07 00:00 [revised]
PHST- 2022/07/11 00:00 [accepted]
PHST- 2022/08/14 06:00 [pubmed]
PHST- 2022/09/21 06:00 [medline]
PHST- 2022/08/13 18:20 [entrez]
AID - S1751-7311(22)00162-8 [pii]
AID - 10.1016/j.animal.2022.100609 [doi]
PST - ppublish
SO  - Animal. 2022 Sep;16(9):100609. doi: 10.1016/j.animal.2022.100609. Epub 2022 Aug 
      11.