PMID- 35966655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220816
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - A novel of new class II bacteriocin from Bacillus velezensis HN-Q-8 and its 
      antibacterial activity on Streptomyces scabies.
PG  - 943232
LID - 10.3389/fmicb.2022.943232 [doi]
LID - 943232
AB  - Potato common scab is a main soil-borne disease of potato that can significantly 
      reduce its quality. At present, it is still a challenge to control potato common 
      scab in the field. To address this problem, the 972 family lactococcin (Lcn972) 
      was screened from Bacillus velezensis HN-Q-8 in this study, and an Escherichia 
      coli overexpression system was used to obtain Lcn972, which showed a significant 
      inhibitory effect on Streptomyces scabies, with a minimum inhibitory 
      concentration of 10.58 mug/mL. The stability test showed that Lcn972 is stable 
      against UV radiation and high temperature. In addition, long-term storage at room 
      temperature and 4 degrees C had limited effects on its activity level. The antibacterial 
      activity of Lcn972 was enhanced by Cu(2+) and Ca(2+), but decreased by protease 
      K. The protein was completely inactivated by Fe(2+). Cell membrane staining 
      showed that Lcn972 damaged the cell membrane integrity of S. scabies. Scanning 
      electron microscope (SEM) and transmission electron microscope (TEM) observations 
      revealed that the hyphae of S. scabies treated with Lcn972 were deformed and 
      adhered, the cell membrane was incomplete, the cytoplasm distribution was uneven, 
      and the cell appeared hollow inside, which led to the death of S. scabies. In 
      conclusion, we used bacteriocin for controlling potato common scab for the first 
      time in this study, and it provides theoretical support for the further 
      application of bacteriocin in the control of plant diseases.
CI  - Copyright (c) 2022 Zhao, Zhou, Bai, Zhang, Zhang, Wang, Wu, Zhu and Yang.
FAU - Zhao, Jing
AU  - Zhao J
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Zhou, Zhijun
AU  - Zhou Z
AD  - Experimental Training Center of Hebei Agricultural University, Baoding, China.
FAU - Bai, Xuefei
AU  - Bai X
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Zhang, Dai
AU  - Zhang D
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Zhang, Likui
AU  - Zhang L
AD  - College of Environmental Science, Yangzhou University, Yangzhou, China.
FAU - Wang, Jinhui
AU  - Wang J
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Wu, Beibei
AU  - Wu B
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Zhu, Jiehua
AU  - Zhu J
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
FAU - Yang, Zhihui
AU  - Yang Z
AD  - College of Plant Protection, Hebei Agricultural University, Baoding, China.
AD  - Technological Innovation Center for Biological Control of Crop Diseases and 
      Insect Pests of Hebei Province, Baoding, China.
LA  - eng
PT  - Journal Article
DEP - 20220729
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9372549
OTO - NOTNLM
OT  - Bacillus
OT  - Lcn972
OT  - Streptomyces scabies
OT  - potato
OT  - potato common scab
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/16 06:00
MHDA- 2022/08/16 06:01
PMCR- 2022/07/29
CRDT- 2022/08/15 03:45
PHST- 2022/05/13 00:00 [received]
PHST- 2022/06/28 00:00 [accepted]
PHST- 2022/08/15 03:45 [entrez]
PHST- 2022/08/16 06:00 [pubmed]
PHST- 2022/08/16 06:01 [medline]
PHST- 2022/07/29 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.943232 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Jul 29;13:943232. doi: 10.3389/fmicb.2022.943232. 
      eCollection 2022.