PMID- 35967565
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240113
IS  - 2296-2360 (Print)
IS  - 2296-2360 (Electronic)
IS  - 2296-2360 (Linking)
VI  - 10
DP  - 2022
TI  - Stability of novel urinary biomarkers used for lupus nephritis.
PG  - 974049
LID - 10.3389/fped.2022.974049 [doi]
LID - 974049
AB  - BACKGROUND: The Renal Activity Index for Lupus (RAIL) is a composite score of six 
      urinary biomarkers (neutrophil gelatinase-associated lipocalin (NGAL), monocyte 
      chemoattractant protein-1 (MCP-1), kidney injury molecule-1 (KIM-1), 
      ceruloplasmin, adiponectin, and hemopexin) used to monitor lupus nephritis 
      activity in children. We tested stability of RAIL biomarkers prior to meaningful 
      clinical use. METHODS: Urine samples were tested by ELISA under shipping 
      conditions, freeze/thaw, ambient and longer-term storage. Statistical analysis 
      was performed via Deming Regression, Bland-Altman and Spearman Correlation 
      Coefficient. RESULTS: Biomarker concentration were comparable to freshly 
      collected urine following storage at -80  degrees C for up to 3 months, and at 4 or 25  degrees C 
      up to 48 h followed by -80  degrees C. Neither shipping on dry or wet ice exposure nor 
      addition of two freeze-thaw cycles led to loss of signal, with excellent Spearman 
      Correlation coefficients under all conditions. CONCLUSIONS: RAIL biomarkers are 
      stable following short-term storage at clinically relevant conditions.
CI  - Copyright (c) 2022 Cody, Rose, Huang, Qiu, Brunner and Devarajan.
FAU - Cody, Ellen M
AU  - Cody EM
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
FAU - Rose, James E
AU  - Rose JE
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
FAU - Huang, Bin
AU  - Huang B
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
AD  - Division of Biostatics and Epidemiology, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
AD  - School of Medicine, University of Cincinnati, Cincinnati, OH, United States.
FAU - Qiu, Tingting
AU  - Qiu T
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
AD  - Division of Biostatics and Epidemiology, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
FAU - Brunner, Hermine I
AU  - Brunner HI
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
AD  - School of Medicine, University of Cincinnati, Cincinnati, OH, United States.
AD  - Division of Rheumatology, Cincinnati Children's Hospital Medical Center, 
      Cincinnati, OH, United States.
FAU - Devarajan, Prasad
AU  - Devarajan P
AD  - Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical 
      Center, Cincinnati, OH, United States.
AD  - School of Medicine, University of Cincinnati, Cincinnati, OH, United States.
LA  - eng
GR  - P30 AR076316/AR/NIAMS NIH HHS/United States
GR  - P50 DK096418/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20220729
PL  - Switzerland
TA  - Front Pediatr
JT  - Frontiers in pediatrics
JID - 101615492
PMC - PMC9372620
OTO - NOTNLM
OT  - SLE
OT  - biomarker
OT  - lupus nephritis
OT  - stability
OT  - urine
COIS- Author PD is a co-inventor on submitted patents for the use of NGAL as a 
      biomarker of kidney disease. The remaining authors declare that the research was 
      conducted in the absence of any commercial or financial relationships that could 
      be construed as a potential conflict of interest.
EDAT- 2022/08/16 06:00
MHDA- 2022/08/16 06:01
PMCR- 2022/07/29
CRDT- 2022/08/15 04:00
PHST- 2022/06/20 00:00 [received]
PHST- 2022/07/11 00:00 [accepted]
PHST- 2022/08/15 04:00 [entrez]
PHST- 2022/08/16 06:00 [pubmed]
PHST- 2022/08/16 06:01 [medline]
PHST- 2022/07/29 00:00 [pmc-release]
AID - 10.3389/fped.2022.974049 [doi]
PST - epublish
SO  - Front Pediatr. 2022 Jul 29;10:974049. doi: 10.3389/fped.2022.974049. eCollection 
      2022.