PMID- 35968589
OWN - NLM
STAT- MEDLINE
DCOM- 20220816
LR  - 20231102
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 43
IP  - 6
DP  - 2022 Jun 14
TI  - [Study of cytogenetics and molecular biology in typical and atypical 
      immunophenotypic chronic lymphocytic leukemia].
PG  - 469-474
LID - 10.3760/cma.j.issn.0253-2727.2022.06.005 [doi]
AB  - Objective: To analyze the differences in immunophenotype, cytogenetics, and 
      molecular biology between typical and atypical immunophenotype chronic 
      lymphocytic leukemia (CLL) , and explore the correlation of cytogenetic anomalies 
      with gene mutations. Methods: This study included 488 patients diagnosed in the 
      First Affiliated Hospital of Nanjing Medical University between November 2014 and 
      May 2021. Of these, 382 patients scored 4-5 points, which was typical CLL (tCLL) 
      , and 106 scored 3 points, which was atypical CLL (aCLL) as per the Royal Marsden 
      Hospital Immunomarker Integral System. Peripheral blood cells were collected for 
      immunophenotype by multiparameter flow cytometry in 488 patients, fluorescence in 
      situ hybridization (FISH) was employed to detect cytogenetic anomalies in 359 
      patients, and gene mutations were detected by next-generation sequencing (NGS) in 
      330 patients. Results: The positive rates of CD10, CD22, CD49d, CD81, and FMC7 
      were significantly higher in the aCLL compared with the tCLL group (P=0.020, 
      P<0.001, P<0.001, P=0.027, and P<0.001, respectively) , while the positive rates 
      of CD5, CD23, CD148, and CD200 were lower in the former compared to the latter 
      (P<0.001, P=0.017, P=0.041, and P<0.001, respectively) . aCLL exhibited a higher 
      frequency of trisomy 12 and lower frequency of del (13q14) compared to the tCLL 
      group (P<0.001 and P<0.001, respectively) . Moreover, aCLL patients also showed a 
      higher incidence of NOTCH1 mutations than the tCLL patients (P=0.038) , while no 
      statistically significant differences in other gene mutations occurred between 
      the two groups. No significant differences in overall survival (OS) and 
      treatment-free survival (TFS) occurred between aCLL and tCLL using Kaplan-Meier 
      analysis (P>0.05) . Conclusion: aCLL has characteristic immunophenotype, 
      cytogenetic, and somatic mutation that differ from tCLL, and this can provide 
      reliable information for the diagnosis and differential diagnosis between the two 
      groups.
FAU - Jin, H M
AU  - Jin HM
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Qiao, C
AU  - Qiao C
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Zhao, S S
AU  - Zhao SS
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Qiu, H R
AU  - Qiu HR
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Chen, X
AU  - Chen X
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Yang, H
AU  - Yang H
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Zhu, L Y
AU  - Zhu LY
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Li, J Y
AU  - Li JY
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
FAU - Wu, Y J
AU  - Wu YJ
AD  - The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province 
      Hospital, Hematology Department, Nanjing 210029, China.
LA  - chi
GR  - 81370656/National Natural Science Foundation of China/
GR  - 2014-WSN-009/Six Talent Peaks Project in Jiangsu Province/
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - Molecular Biology
PMC - PMC9800222
OTO - NOTNLM
OT  - Cytogenetics
OT  - Immunophenotyping
OT  - Leukemia, lymphocytic, chronic
OT  - Molecular biology
COIS- 利益冲突 所有作者声明无利益冲突
EDAT- 2022/08/16 06:00
MHDA- 2022/08/17 06:00
PMCR- 2022/06/01
CRDT- 2022/08/15 04:18
PHST- 2022/08/15 04:18 [entrez]
PHST- 2022/08/16 06:00 [pubmed]
PHST- 2022/08/17 06:00 [medline]
PHST- 2022/06/01 00:00 [pmc-release]
AID - cjh-43-06-469 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2022.06.005 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2022 Jun 14;43(6):469-474. doi: 
      10.3760/cma.j.issn.0253-2727.2022.06.005.