PMID- 35971749
OWN - NLM
STAT- MEDLINE
DCOM- 20221012
LR  - 20221012
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 46
IP  - 11
DP  - 2022 Nov
TI  - CircSERPINA3 promoted cell proliferation, migration, and invasion of laryngeal 
      squamous cell carcinoma by targeting miR-885-5p.
PG  - 1852-1863
LID - 10.1002/cbin.11872 [doi]
AB  - CircSERPINA3 has been shown to be upregulated in laryngeal squamous cell 
      carcinoma (LSCC); however, whether it regulates the development of LSCC and the 
      specific molecular mechanism remains unclear, which is to be explored in this 
      study. Expressions of circSERPINA3, miR-885-5p, and Malic enzyme 1 (ME1) in LSCC 
      tissues or cell lines were determined by quantitative reverse transcription 
      polymerase chain reaction (qRT-PCR). The regulation of circSERPINA3 on the 
      biological behavior of LSCC cells was confirmed by loss and gain experiments 
      (cell counting kit-8, transwell, and colony formation assay). The correlation 
      between circSERPINA3/ME1 and miR-885-5p was predicted and confirmed by 
      bioinformatics analysis, dual-luciferase reporter assay, and qRT-PCR. The effect 
      of circSERPINA3/miR-885-5p axis on the biological behavior of LSCC cells and 
      expressions of epithelial-mesenchymal transition-related proteins was confirmed 
      by rescue experiments. CircSERPINA3 and ME1 was upregulated in LSCC tissues, 
      whereas miR-885-5p was downregulated. MiR-885-5p was the target gene of 
      circSERPINA3, whereas ME1 was the target gene of miR-885-5p. Silent circSERPINA3 
      suppressed viability, invasion, migration, colony formation, and expression of 
      ME1, claudin-4, snail, and vimentin but elevated expression of miR-885-5p and 
      E-cadherin, whereas overexpressed circSERPINA3 was the opposite. However, 
      miR-885-5p inhibitor or mimic reversed the effects of silent circSERPINA3 or 
      overexpressed circSERPINA3. Collectively, circSERPINA3 promotes proliferation, 
      migration, and invasion of LSCC cells by targeting miR-885-5p.
CI  - (c) 2022 International Federation for Cell Biology.
FAU - Xu, Qiushi
AU  - Xu Q
AD  - Ear Nose and Throat Department, The First Hospital of Qiqihar, Affiliated Qiqihar 
      Hospital, Southern Medical University, Qiqihar, China.
FAU - Yu, Bing
AU  - Yu B
AD  - Pathology Department, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, 
      Southern Medical University, Qiqihar, China.
FAU - Chen, Wenjing
AU  - Chen W
AD  - Pathology Department, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, 
      Southern Medical University, Qiqihar, China.
FAU - Li, Wenlong
AU  - Li W
AD  - Ear Nose and Throat Department, The First Hospital of Qiqihar, Affiliated Qiqihar 
      Hospital, Southern Medical University, Qiqihar, China.
FAU - Sun, Yuanhao
AU  - Sun Y
AD  - Ear Nose and Throat Department, The First Hospital of Qiqihar, Affiliated Qiqihar 
      Hospital, Southern Medical University, Qiqihar, China.
FAU - Fang, Yanchun
AU  - Fang Y
AUID- ORCID: 0000-0003-3684-3483
AD  - Pathology Department, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, 
      Southern Medical University, Qiqihar, China.
LA  - eng
PT  - Journal Article
DEP - 20220816
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Cadherins)
RN  - 0 (Claudin-4)
RN  - 0 (MIRN885 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Vimentin)
SB  - IM
MH  - Cadherins/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Claudin-4/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Vimentin/metabolism
OTO - NOTNLM
OT  - circSERPINA3
OT  - laryngeal squamous cell carcinoma
OT  - malic enzyme 1
OT  - miR-885-5p
EDAT- 2022/08/17 06:00
MHDA- 2022/10/13 06:00
CRDT- 2022/08/16 03:43
PHST- 2022/02/16 00:00 [received]
PHST- 2022/07/07 00:00 [accepted]
PHST- 2022/08/17 06:00 [pubmed]
PHST- 2022/10/13 06:00 [medline]
PHST- 2022/08/16 03:43 [entrez]
AID - 10.1002/cbin.11872 [doi]
PST - ppublish
SO  - Cell Biol Int. 2022 Nov;46(11):1852-1863. doi: 10.1002/cbin.11872. Epub 2022 Aug 
      16.