PMID- 35972226
OWN - NLM
STAT- MEDLINE
DCOM- 20221121
LR  - 20230817
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Print)
IS  - 0305-7364 (Linking)
VI  - 130
IP  - 5
DP  - 2022 Nov 17
TI  - Network of the transcriptome and metabolomics reveals a novel regulation of 
      drought resistance during germination in wheat.
PG  - 717-735
LID - 10.1093/aob/mcac102 [doi]
AB  - BACKGROUND AND AIMS: The North China Plain, the highest winter-wheat-producing 
      region of China, is seriously threatened by drought. Traditional irrigation 
      wastes a significant amount of water during the sowing season. Therefore, it is 
      necessary to study the drought resistance of wheat during germination to maintain 
      agricultural ecological security. From several main cultivars in the North China 
      Plain, we screened the drought-resistant cultivar JM47 and drought-sensitive 
      cultivar AK58 during germination using the polyethylene glycol (PEG) drought 
      simulation method. An integrated analysis of the transcriptome and metabolomics 
      was performed to understand the regulatory networks related to drought resistance 
      in wheat germination and verify key regulatory genes. METHODS: Transcriptional 
      and metabolic changes were investigated using statistical analyses and 
      gene-metabolite correlation networks. Transcript and metabolite profiles were 
      obtained through high-throughput RNA-sequencing data analysis and 
      ultra-performance liquid chromatography quadrupole time-of-flight tandem mass 
      spectrometry, respectively. KEY RESULTS: A total of 8083 and 2911 differentially 
      expressed genes (DEGs) and 173 and 148 differential metabolites were identified 
      in AK58 and JM47, respectively, under drought stress. According to the integrated 
      analysis results, mammalian target of rapamycin (mTOR) signalling was prominently 
      enriched in JM47. A decrease in alpha-linolenic acid content was consistent with the 
      performance of DEGs involved in jasmonic acid biosynthesis in the two cultivars 
      under drought stress. Abscisic acid (ABA) content decreased more in JM47 than in 
      AK58, and linoleic acid content decreased in AK58 but increased in JM47. 
      alpha-Tocotrienol was upregulated and strongly correlated with alpha-linolenic acid 
      metabolism. CONCLUSIONS: The DEGs that participated in the mTOR and alpha-linolenic 
      acid metabolism pathways were considered candidate DEGs related to drought 
      resistance and the key metabolites alpha-tocotrienol, linoleic acid and l-leucine, 
      which could trigger a comprehensive and systemic effect on drought resistance 
      during germination by activating mTOR-ABA signalling and the interaction of 
      various hormones.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the 
      Annals of Botany Company. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Li, Zongzhen
AU  - Li Z
AUID- ORCID: 0000-0003-4111-1826
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Lian, Yanhao
AU  - Lian Y
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Gong, Pu
AU  - Gong P
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Song, Linhu
AU  - Song L
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Hu, Junjie
AU  - Hu J
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Pang, Haifang
AU  - Pang H
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Ren, Yongzhe
AU  - Ren Y
AUID- ORCID: 0000-0002-7372-7775
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Xin, Zeyu
AU  - Xin Z
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Wang, Zhiqiang
AU  - Wang Z
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
FAU - Lin, Tongbao
AU  - Lin T
AD  - College of Agronomy, Henan Agricultural University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 0RBV727H71 (alpha-Linolenic Acid)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - *Droughts
MH  - *Triticum/physiology
MH  - Germination
MH  - Transcriptome
MH  - alpha-Linolenic Acid/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Linoleic Acid/metabolism
MH  - Metabolomics
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Stress, Physiological/genetics
MH  - Gene Expression Profiling
PMC - PMC9670757
OTO - NOTNLM
OT  - Abscisic acid
OT  - RNA-seq
OT  - drought resistance
OT  - germination
OT  - linoleic acid
OT  - mTOR signalling
OT  - metabolomics
OT  - wheat
OT  - alpha-linolenic acid
EDAT- 2022/08/17 06:00
MHDA- 2022/11/22 06:00
PMCR- 2023/08/16
CRDT- 2022/08/16 08:53
PHST- 2022/07/10 00:00 [received]
PHST- 2022/08/13 00:00 [accepted]
PHST- 2022/08/17 06:00 [pubmed]
PHST- 2022/11/22 06:00 [medline]
PHST- 2022/08/16 08:53 [entrez]
PHST- 2023/08/16 00:00 [pmc-release]
AID - 6668258 [pii]
AID - mcac102 [pii]
AID - 10.1093/aob/mcac102 [doi]
PST - ppublish
SO  - Ann Bot. 2022 Nov 17;130(5):717-735. doi: 10.1093/aob/mcac102.