PMID- 35973349
OWN - NLM
STAT- MEDLINE
DCOM- 20220928
LR  - 20221003
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 101
IP  - 10
DP  - 2022 Oct
TI  - Research Note: Development and characterization of monoclonal antibodies specific 
      for chicken interleukin-7 receptor alpha (CD127).
PG  - 102047
LID - S0032-5791(22)00338-8 [pii]
LID - 10.1016/j.psj.2022.102047 [doi]
LID - 102047
AB  - CD127, also named interleukin-7 receptor (IL-7R), is expressed on various cell 
      types including naive and memory T cells, and plays a critical role in the 
      differentiation and activation of T lymphocytes. The availability of 
      poultry-specific immune reagents to identify and measure chicken CD127 response 
      will enhance fundamental and applied research in poultry immunology. Mouse 
      monoclonal antibodies (MAbs) against chicken CD127 (chCD127) were developed and 
      characterized. More specifically, a 678 bp ectodomain of chCD127 gene was cloned 
      in the pET28a (+) vector and expressed in BL21-AI E. coli competent cells. The 
      recombinant chCD127 protein with a size of 30 KDa which was also recognized by a 
      mouse anti-human CD127 MAb (Clone G-11) was used to immunize mice, and 6 new 
      mouse MAbs which specifically detected chicken CD127 were developed and 
      characterized. Availability of these new sets of chCD127-specific MAbs will 
      facilitate the immunological studies on CD127 in poultry, especially in 
      understanding effector and memory T immune cell responses in normal and diseased 
      states.
CI  - Published by Elsevier Inc.
FAU - Sun, Zhifeng
AU  - Sun Z
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA.
FAU - Lu, Mingmin
AU  - Lu M
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA.
FAU - Kim, Woohyun
AU  - Kim W
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA.
FAU - Lillehoj, Hyun
AU  - Lillehoj H
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA.
FAU - Panebra, Alfredo
AU  - Panebra A
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA.
FAU - Zhao, Hongyan
AU  - Zhao H
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA; College of 
      Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
FAU - Li, Charles
AU  - Li C
AD  - Animal Bioscience and Biotechnology Laboratory, Beltsville Agriculture Research 
      Center, Agricultural Research Service, USDA, Beltsville, MD 20705 USA. Electronic 
      address: charles.li@usda.gov.
LA  - eng
PT  - Journal Article
DEP - 20220709
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Interleukin-7)
RN  - 0 (Interleukin-7 Receptor alpha Subunit)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - *Chickens/genetics
MH  - Escherichia coli
MH  - Interleukin-7
MH  - Interleukin-7 Receptor alpha Subunit/*immunology
MH  - Mice
MH  - Recombinant Proteins
PMC - PMC9396400
OTO - NOTNLM
OT  - CD127
OT  - IL-7Ra
OT  - chicken
OT  - monoclonal antibody
OT  - thymocyte proliferation inhibition
EDAT- 2022/08/17 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/07/09
CRDT- 2022/08/16 18:22
PHST- 2022/03/08 00:00 [received]
PHST- 2022/06/28 00:00 [revised]
PHST- 2022/06/29 00:00 [accepted]
PHST- 2022/08/17 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/08/16 18:22 [entrez]
PHST- 2022/07/09 00:00 [pmc-release]
AID - S0032-5791(22)00338-8 [pii]
AID - 102047 [pii]
AID - 10.1016/j.psj.2022.102047 [doi]
PST - ppublish
SO  - Poult Sci. 2022 Oct;101(10):102047. doi: 10.1016/j.psj.2022.102047. Epub 2022 Jul 
      9.