PMID- 35974282
OWN - NLM
STAT- MEDLINE
DCOM- 20230206
LR  - 20230206
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 30
IP  - 2
DP  - 2023 Jan
TI  - Whole cell of pure Clostridium butyricum CBT-1 from anaerobic bioreactor 
      effectively hydrolyzes agro-food waste into biohydrogen.
PG  - 4853-4865
LID - 10.1007/s11356-022-22443-9 [doi]
AB  - Recycling organic waste and converting them into renewable energy are a promising 
      route for environment protection and effective biochemical reactions suitable for 
      industrial hydrogen synthesis. This study targeted to isolate a pure anaerobic 
      culture with potential to hydrolyze different biomass and production of 
      biohydrogen. For this, a sample of full-scale anaerobic digester, fed with a 
      multicomponent solid, was inoculated on Reinforced Clostridial Medium (RCM) in 
      strict anaerobic conditions. An anaerobic Clostridium butyricum CBT-1 strain was 
      isolated, identified from morphological and 16S rRNA sequence. The CBT-1 culture 
      expressed amylase, cellulase and peroxidases activities. The strain exhibited 
      visual decolorization of both Azure B and crystal violet dyes. In batch 
      fermentation experiment, the CBT-1 produced highest of 3.06, 2.67 and 2.46 
      mol/mol H(2) yield from glucose, starch and cellulose respectively, whereas, the 
      CBT-1 showed low 0.43 mol H(2)/mol of substrate from untreated rice straw due to 
      lignin in compact structure and comparatively high H(2) yield of 1.91 and 2.01 
      mol H(2)/mol of substrate rice straw hydrolysate and kitchen food waste (KFWS) 
      respectively. The cumulative volumetric yield of H(2) was 358.15, 300.8 and 
      294.5NmL/gSub from glucose, starch and cellulose respectively. Similarly, the 
      cumulative H(2) volume was 76.7, 184.4, 237.2 NmL/gVS from untreated rice straw, 
      rice straw hydrolysate and kitchen food waste. This study emphasizes the 
      prospects to find similar robust anaerobic culture for hydrolyzing complex 
      biomass. Such strains could be used as standard co-inoculum for biohydrogen 
      obtaining and as the biocatalyst for commercial scale applications.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Shah, Tawaf Ali
AU  - Shah TA
AUID- ORCID: 0000-0002-5030-4729
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China. tawafbiotech@yahoo.com.
FAU - Zhihe, Li
AU  - Zhihe L
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
FAU - Zhiyu, Li
AU  - Zhiyu L
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
FAU - Zhang, Andong
AU  - Zhang A
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
FAU - Lu, Di
AU  - Lu D
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
FAU - Fang, Wang
AU  - Fang W
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
FAU - Xuan, Han
AU  - Xuan H
AD  - Department of Agriculture Engineering and Food Science, Shandong University of 
      Technology, Zibo, China.
LA  - eng
PT  - Journal Article
DEP - 20220817
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9004-34-6 (Cellulose)
RN  - 9005-25-8 (Starch)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - *Clostridium butyricum/genetics/metabolism
MH  - Anaerobiosis
MH  - Food
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Refuse Disposal
MH  - Bioreactors
MH  - Fermentation
MH  - Cellulose
MH  - Starch
MH  - Hydrogen
OTO - NOTNLM
OT  - Bioenergy
OT  - Culture
OT  - Fermentation
OT  - Renewable
OT  - Volume
OT  - Waste degradation
EDAT- 2022/08/17 06:00
MHDA- 2023/02/07 06:00
CRDT- 2022/08/16 23:35
PHST- 2022/03/02 00:00 [received]
PHST- 2022/08/04 00:00 [accepted]
PHST- 2022/08/17 06:00 [pubmed]
PHST- 2023/02/07 06:00 [medline]
PHST- 2022/08/16 23:35 [entrez]
AID - 10.1007/s11356-022-22443-9 [pii]
AID - 10.1007/s11356-022-22443-9 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2023 Jan;30(2):4853-4865. doi: 
      10.1007/s11356-022-22443-9. Epub 2022 Aug 17.