PMID- 35976169
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20230313
IS  - 2194-802X (Electronic)
IS  - 2194-802X (Linking)
VI  - 9
IP  - 4
DP  - 2022 Nov 1
TI  - miR-21-3p and miR-192-5p in patients with type 2 diabetic nephropathy.
PG  - 499-507
LID - 10.1515/dx-2022-0036 [doi]
AB  - OBJECTIVES: Microribonucleic acids (microRNA/miRNA/miR-) are predicted to be 
      useful in the early diagnosis, monitoring, and treatment of diabetic nephropathy 
      (DN). We aimed to investigate the relationship of DN to miR-21-3p, miR-29a-3p, 
      miR-29b-3p, miR-29c-3p, miR-126-3p, miR-129-1-3p, miR-137, miR-192-5p, 
      miR-212-3p, and miR-320c. METHODS: There were 50 healthy controls and 100 
      patients with type 2 diabetes mellitus (T2DM). The diabetic patients were divided 
      into three subgroups: normal to mildly increased (A1, n=51), moderately increased 
      (A2, n=25), and severely increased (A3, n=24) albuminuria. The biochemical 
      measurements were analysed using Roche Cobas 8000. The plasma miRNAs were 
      analysed using RT-qPCR based on SYBR green chemistry. RESULTS: The relative 
      expression of miR-21-3p was significantly lower in the (A3 p=0.005, 6.6-fold 
      decrease) and DN (A1 + A3) (p=0.005, 6.6-fold decrease) groups compared to the 
      controls. The relative expression of miR-192-5p was also significantly lower in 
      the DN group (p=0.027, 2.4-fold decrease) compared to the controls. The area 
      under curve value was 0.726 for miR-21-3p and 0.717 for miR-192-5p for 
      distinguishing the DN group from the controls. CONCLUSIONS: The decreased 
      expressions of miR-21-3p and miR-192-5p are associated with the development of DN 
      and may be potential biomarkers for the early diagnosis of DN.
CI  - (c) 2022 Walter de Gruyter GmbH, Berlin/Boston.
FAU - Akpinar, Kadriye
AU  - Akpinar K
AUID- ORCID: 0000-0002-6951-8866
AD  - Department of Medical Biochemistry, School of Medicine, Pamukkale University, 
      Denizli, Turkey.
FAU - Aslan, Diler
AU  - Aslan D
AUID- ORCID: 0000-0003-4907-9445
AD  - Department of Medical Biochemistry, School of Medicine, Pamukkale University, 
      Denizli, Turkey.
FAU - Fenkci, Semin Melahat
AU  - Fenkci SM
AUID- ORCID: 0000-0002-5658-459X
AD  - Department of Internal Medicine, Division of Endocrinology and Metabolism, School 
      of Medicine, Pamukkale University, Denizli, Turkey.
FAU - Caner, Vildan
AU  - Caner V
AUID- ORCID: 0000-0003-0980-9335
AD  - Department of Medical Genetics, School of Medicine, Pamukkale University, 
      Denizli, Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220818
PL  - Germany
TA  - Diagnosis (Berl)
JT  - Diagnosis (Berlin, Germany)
JID - 101654734
RN  - 0 (MicroRNAs)
RN  - 0 (Biomarkers)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (MIRN137 microRNA, human)
RN  - 0 (MIRN192 microRNA, human)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MIRN212 microRNA, human)
SB  - IM
MH  - Humans
MH  - *Diabetic Nephropathies/diagnosis/genetics
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - *MicroRNAs
MH  - Albuminuria/diagnosis/genetics
MH  - Biomarkers
OTO - NOTNLM
OT  - circulating microRNAs
OT  - diabetes mellitus
OT  - diabetic nephropathy
EDAT- 2022/08/18 06:00
MHDA- 2022/11/16 06:00
CRDT- 2022/08/17 10:33
PHST- 2022/04/19 00:00 [received]
PHST- 2022/07/23 00:00 [accepted]
PHST- 2022/08/18 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
PHST- 2022/08/17 10:33 [entrez]
AID - dx-2022-0036 [pii]
AID - 10.1515/dx-2022-0036 [doi]
PST - epublish
SO  - Diagnosis (Berl). 2022 Aug 18;9(4):499-507. doi: 10.1515/dx-2022-0036. 
      eCollection 2022 Nov 1.