PMID- 35979793
OWN - NLM
STAT- MEDLINE
DCOM- 20220819
LR  - 20220821
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 36
IP  - 8
DP  - 2022 Aug 15
TI  - [Study on bone microstructure and pathology of type L2 and L3 osteonecrosis of 
      the femoral head specimens classified by China-Japan Friendship Hospital 
      classification based on "three-columns structure"].
PG  - 1003-1010
LID - 10.7507/1002-1892.202203108 [doi]
AB  - OBJECTIVE: To analyze the similarities and differences of bone microstructure and 
      apoptosis phenotype of lateral column, middle column, and medial column in type 
      L2 and L3 osteonecrosis of the femoral head (ONFH) specimens classified by 
      China-Japan Friendship Hospital (CJFH) classification, so as to carry out a 
      quantitative study of ONFH "three-columns structure theory" and to provide 
      research support for the treatment of ONFH by rotational osteotomy through the 
      base of femoral neck. METHODS: Discarded femoral head specimens from 16 patients 
      (16 hips) with CJFH type L2 and L3 ONFH undergone total hip arthroplasty between 
      April 2020 and February 2021 were selected as the research objects. First, the 
      "three-column structure" of the femoral head was three-dimensionally segmented by 
      Micro-CT, and the bone volume to total volume (BV/TV), bone surface area to bone 
      volume ratio (BS/BV), trabecular spacing/separation (Tb.Sp), trabecular thickness 
      (Tb.Th), and trabecular number (Tb.N) in the lateral column, middle column, and 
      medial column were analyzed to compare the similarities and differences of 
      parameters related to bone mass and trabecular structure among the three columns. 
      Then, the specimens were cut with a oscillating saw and made into paraffin 
      sections for HE staining and immunohistochemical staining of B-cell lymphoma-2 
      (Bcl-2) and Bcl-2 associated X protein (Bax). The differences of apoptosis 
      phenotype between the three columns of ONFH samples of CJFH type L2 and L3 were 
      evaluated by comparing the rate of empty lacunae and the rate of positive cells 
      of immunohistochemical staining. RESULTS: There were significant differences in 
      BV/TV, Tb.Th, and Tb.N among the three columns of CJFH type L2 ONFH femoral head 
      ( P<0.05), with the largest in the medial column and the smallest in the lateral 
      column; BS/BV and Tb.Sp of the lateral column were significantly greater than 
      those of the medial column and middle column ( P<0.05), no significant difference 
      was found between the middle column and medial column ( P>0.05). There was no 
      significant difference in the bone parameters between the three columns of the 
      CJFH type L3 ONFH femoral head ( P>0.05). There was no significant difference in 
      the rate of empty lacunae between the three columns of the CJFH type L2 and L3 
      ONFH femoral head ( P>0.05). Immunohistochemical staining showed that a large 
      number of tissue sections were detached, and only a small amount of non-specific 
      staining was found in the sections without detachment, so the positive cell rate 
      could not be calculated. CONCLUSION: The middle and medial columns of the CJFH 
      type L2 ONFH has better trabecular structures than the lateral column, and there 
      is no significant difference in trabecular structures among the three columns of 
      the CJFH type L3 ONFH.
FAU - Gao, Runzi
AU  - Gao R
AD  - Department of Orthopedics and Traumatology, Nanjing Hospital of Chinese Medicine 
      Affiliated to Nanjing University of Chinese Medicine, Nanjing Jiangsu, 210029, P. 
      R. China.
FAU - Xia, Xiawei
AU  - Xia X
AD  - Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing 
      University of Chinese Medicine, Nanjing Jiangsu, 210001, P. R. China.
FAU - Liu, Jinzhu
AU  - Liu J
AD  - Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing 
      University of Chinese Medicine, Nanjing Jiangsu, 210001, P. R. China.
FAU - Yuan, Zhaofeng
AU  - Yuan Z
AD  - Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing 
      University of Chinese Medicine, Nanjing Jiangsu, 210001, P. R. China.
FAU - Huan, Dawei
AU  - Huan D
AD  - Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing 
      University of Chinese Medicine, Nanjing Jiangsu, 210001, P. R. China.
FAU - Shen, Jirong
AU  - Shen J
AD  - Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing 
      University of Chinese Medicine, Nanjing Jiangsu, 210001, P. R. China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - China
MH  - Femur Head/pathology
MH  - *Femur Head Necrosis/surgery
MH  - Friends
MH  - Hospitals
MH  - Humans
MH  - Japan
MH  - *Osteonecrosis
MH  - Proto-Oncogene Proteins c-bcl-2
PMC - PMC9379465
OTO - NOTNLM
OT  - China Japan Friendship Hospital classification
OT  - Micro-CT
OT  - Osteonecrosis of the femoral head
OT  - pathology
COIS- 利益冲突　在课题研究和文章撰写过程中不存在利益冲突；基金项目经费支持没有影响文章观点和对研究数据客观结果的统计分析及其报道
EDAT- 2022/08/19 06:00
MHDA- 2022/08/20 06:00
PMCR- 2022/08/01
CRDT- 2022/08/18 04:43
PHST- 2022/08/18 04:43 [entrez]
PHST- 2022/08/19 06:00 [pubmed]
PHST- 2022/08/20 06:00 [medline]
PHST- 2022/08/01 00:00 [pmc-release]
AID - zgxfcjwkzz-36-8-1003 [pii]
AID - 10.7507/1002-1892.202203108 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2022 Aug 15;36(8):1003-1010. doi: 
      10.7507/1002-1892.202203108.