PMID- 35980581
OWN - NLM
STAT- MEDLINE
DCOM- 20220822
LR  - 20220914
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2540
DP  - 2022
TI  - Anchor Away: A System for Fast Inhibition of Proteins in Drosophila.
PG  - 239-249
LID - 10.1007/978-1-0716-2541-5_11 [doi]
AB  - Anchor away is a sequestering method designed to acutely and timely abrogate the 
      function of a protein of interest by anchoring to a cell compartment different 
      from its target. This method induces the binding of the target protein to the 
      anchor by either the addition of rapamycin to Drosophila food or cell media. 
      Rapamycin mediates the formation of a ternary complex between the anchor, which 
      is tagged with the FK506-binding protein (FKBP12), and the target protein fused 
      with the FKB12 rapamycin-binding (FRB) domain of mammalian target of rapamycin 
      (mTOR). The rapamycin-bound target protein stays sequestered away from its 
      compartment, where it cannot perform its biological function.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Sanchez Bosch, Pablo
AU  - Sanchez Bosch P
AD  - Stanford University School of Medicine, Stanford, CA, USA. pablosb@stanford.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 5.2.1.- (Tacrolimus Binding Protein 1A)
RN  - EC 5.2.1.- (Tacrolimus Binding Proteins)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - *Drosophila/metabolism
MH  - Mammals/metabolism
MH  - *Sirolimus/pharmacology
MH  - Tacrolimus Binding Protein 1A/metabolism
MH  - Tacrolimus Binding Proteins
OTO - NOTNLM
OT  - CRISPR
OT  - Loss of function
OT  - Protein knockout
OT  - Rapamycin
OT  - TOR
EDAT- 2022/08/19 06:00
MHDA- 2022/08/23 06:00
CRDT- 2022/08/18 11:21
PHST- 2022/08/18 11:21 [entrez]
PHST- 2022/08/19 06:00 [pubmed]
PHST- 2022/08/23 06:00 [medline]
AID - 10.1007/978-1-0716-2541-5_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2540:239-249. doi: 10.1007/978-1-0716-2541-5_11.