PMID- 35982115
OWN - NLM
STAT- MEDLINE
DCOM- 20220822
LR  - 20221026
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Aug 18
TI  - Effectiveness of ozone generated by a dielectric barrier discharge plasma reactor 
      against multidrug-resistant pathogens and Clostridioides difficile spores.
PG  - 14118
LID - 10.1038/s41598-022-18428-w [doi]
LID - 14118
AB  - The contaminated healthcare environment plays an important role in the spread of 
      multidrug-resistant organisms (MDROs) and Clostridioides difficile. This study 
      aimed to evaluate the antimicrobial effects of ozone generated by a dielectric 
      barrier discharge (DBD) plasma reactor on various materials that were 
      contaminated by vancomycin-resistant Enterococcus faecium (VRE), 
      carbapenem-resistant Klebsiella pneumoniae (CRE), carbapenem-resistant 
      Pseudomonas aeruginosa (CRPA), carbapenem-resistant Acinetobacter baumannii 
      (CRAB) and C. difficile spores. Various materials contaminated by VRE, CRE, CRPA, 
      CRAB and C. difficile spores were treated with different ozone concentrations and 
      exposure times. Atomic force microscopy (AFM) demonstrated bacterial surface 
      modifications following ozone treatment. When an ozone dosage of 500 ppm for 
      15 min was applied to VRE and CRAB, about 2 or more log(10) reduction was 
      observed in stainless steel, fabric and wood, and a 1-2 log(10) reduction in 
      glass and plastic. Spores of C. difficile were more resistant to ozone than were 
      all other tested organisms. On AFM, the bacterial cells, following ozone 
      treatment, were swollen and distorted. The ozone generated by the DBD plasma 
      reactor provided a simple and valuable decontamination tool for the MDROs and C. 
      difficile spores, which are known as common pathogens in healthcare-associated 
      infections.
CI  - (c) 2022. The Author(s).
FAU - Bong, Cheolwoo
AU  - Bong C
AD  - Sungkyunkwan University School of Mechanical Engineering, 2066, Serbu-ro, 
      Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
FAU - Choi, Ji Young
AU  - Choi JY
AD  - Department of Microbiology, Sungkyunkwan University School of Medicine, Suwon, 
      South Korea.
FAU - Bae, Jinseung
AU  - Bae J
AD  - Sungkyunkwan University School of Mechanical Engineering, 2066, Serbu-ro, 
      Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
FAU - Park, Sungsu
AU  - Park S
AD  - Sungkyunkwan University School of Mechanical Engineering, 2066, Serbu-ro, 
      Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
AD  - Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 
      Suwon, Korea.
FAU - Ko, Kwan Soo
AU  - Ko KS
AD  - Department of Microbiology, Sungkyunkwan University School of Medicine, Suwon, 
      South Korea.
FAU - Bak, Moon Soo
AU  - Bak MS
AUID- ORCID: 0000-0003-2538-3186
AD  - Sungkyunkwan University School of Mechanical Engineering, 2066, Serbu-ro, 
      Jangan-gu, Suwon-si, Gyeonggi-do, Republic of Korea. moonsoo@skku.edu.
AD  - Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 
      Suwon, Korea. moonsoo@skku.edu.
FAU - Cheong, Hae Suk
AU  - Cheong HS
AUID- ORCID: 0000-0001-8702-1987
AD  - Division of Infectious Diseases, Department of Internal Medicine, Kangbuk Samsung 
      Hospital, Sungkyunkwan University School of Medicine, 29, Saemoonan-ro, 
      Jongro-gu, Seoul, 03181, Republic of Korea. philliper@naver.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220818
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Carbapenems)
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - *Acinetobacter baumannii
MH  - Carbapenems/pharmacology
MH  - Clostridioides
MH  - *Clostridioides difficile
MH  - *Ozone/pharmacology
MH  - Pseudomonas aeruginosa
MH  - Spores, Bacterial
MH  - *Vancomycin-Resistant Enterococci
PMC - PMC9388508
COIS- The authors declare no competing interests.
EDAT- 2022/08/19 06:00
MHDA- 2022/08/23 06:00
PMCR- 2022/08/18
CRDT- 2022/08/18 23:21
PHST- 2022/03/18 00:00 [received]
PHST- 2022/08/11 00:00 [accepted]
PHST- 2022/08/18 23:21 [entrez]
PHST- 2022/08/19 06:00 [pubmed]
PHST- 2022/08/23 06:00 [medline]
PHST- 2022/08/18 00:00 [pmc-release]
AID - 10.1038/s41598-022-18428-w [pii]
AID - 18428 [pii]
AID - 10.1038/s41598-022-18428-w [doi]
PST - epublish
SO  - Sci Rep. 2022 Aug 18;12(1):14118. doi: 10.1038/s41598-022-18428-w.