PMID- 3598213
OWN - NLM
STAT- MEDLINE
DCOM- 19870804
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 156
IP  - 1
DP  - 1987 Jul
TI  - Binding of toxic-shock-syndrome toxin-1 to human peripheral blood mononuclear 
      cells.
PG  - 122-9
AB  - Toxic-shock-syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus and 
      associated with toxic shock syndrome, functions in vitro as both a 
      lymphoproliferative and immunosuppressive protein for human peripheral blood 
      mononuclear cells (PBMs). We analyzed TSST-1-target cell interactions by 
      receptor-ligand binding analyses. In competitive binding experiments, 2 X 10(5) 
      human PBMs or purified cell populations were incubated in the presence of small 
      amounts of (5-50 ng) of 125I-labeled TSST-1 and increasing amounts of unlabeled 
      TSST-1 (25-10,000 ng). Data were analyzed by the method of Scatchard. 
      Toxin-specific receptors were shown to exist on T lymphocytes within the PBM 
      population. T4+ cells had 27.5 X 10(6) receptors per cell, and T8+ cells had 9 X 
      10(6) receptors per cell. T4+ and T8+ receptors had dissociation constants of 
      2.58 X 10(-8) M and 1.8 X 10(-8) M, respectively. These studies confirm earlier 
      work showing that TSST-1 causes the functional activation of a population of T 
      lymphocytes involved in suppression of immunoglobulin responses.
FAU - Poindexter, N J
AU  - Poindexter NJ
FAU - Schlievert, P M
AU  - Schlievert PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - *Bacterial Toxins
MH  - Binding, Competitive
MH  - Concanavalin A/pharmacology
MH  - Enterotoxins/*metabolism/toxicity
MH  - Humans
MH  - In Vitro Techniques
MH  - Iodine Radioisotopes
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocytes/drug effects/*metabolism
MH  - Monocytes/*metabolism
MH  - *Superantigens
MH  - Temperature
EDAT- 1987/07/01 00:00
MHDA- 1987/07/01 00:01
CRDT- 1987/07/01 00:00
PHST- 1987/07/01 00:00 [pubmed]
PHST- 1987/07/01 00:01 [medline]
PHST- 1987/07/01 00:00 [entrez]
AID - 10.1093/infdis/156.1.122 [doi]
PST - ppublish
SO  - J Infect Dis. 1987 Jul;156(1):122-9. doi: 10.1093/infdis/156.1.122.