PMID- 35982489
OWN - NLM
STAT- MEDLINE
DCOM- 20220822
LR  - 20220825
IS  - 1477-7819 (Electronic)
IS  - 1477-7819 (Linking)
VI  - 20
IP  - 1
DP  - 2022 Aug 19
TI  - Effect of Notch1 signaling on cellular proliferation and apoptosis in human 
      laryngeal carcinoma.
PG  - 262
LID - 10.1186/s12957-022-02728-6 [doi]
LID - 262
AB  - BACKGROUND: The occurrence and development of malignancies include excessive 
      proliferation and apoptosis resistance in tumor cells. This study aimed to 
      identify the effects of Notch1 signaling on proliferation and apoptosis of 
      laryngeal cancer cells in a hypoxic microenvironment. METHODS: Notch1 and Ki-67 
      expression in laryngeal squamous cell carcinoma (LSCC) tissues was detected by 
      immunohistochemistry. The apoptotic index (AI) of LSCC was evaluated by the TUNEL 
      method. Small interfering RNA (siRNA) was used to inhibit Notch1 expression in 
      laryngeal cancer cells. Real-time PCR was used to measure Notch1, Hes1, and Hey1 
      mRNA expression, and Western blotting was used to measure Notch1 and Notch1 
      intracellular domain (N1ICD) protein expression. Annexin V-FITC/propidium iodide 
      staining and Cell Counting Kit-8 assays were used to measure cell apoptosis and 
      proliferation, respectively. RESULTS: Notch1 expression was significantly related 
      to the proliferation index (PI) and AI in LSCC tissues. Hypoxia could induce 
      proliferation and inhibit apoptosis in cancer cells. Notch1 expression and Notch1 
      signaling activity could be upregulated by hypoxia. Suppressing Notch1 signaling 
      activity in hypoxic cells could decrease proliferation and increase apoptosis. 
      CONCLUSIONS: Our study has demonstrated that hypoxia may promote proliferation 
      and inhibit apoptosis of laryngeal cancer cells. Notch1 signaling may play a 
      pivotal role in regulating the proliferation and apoptosis resistance of 
      laryngeal cancer cells under hypoxic conditions.
CI  - (c) 2022. The Author(s).
FAU - Li, Dawei
AU  - Li D
AD  - Department of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai 
      Jiaotong University School of Medicine; Shanghai Jiaotong University School of 
      Medicine Ear Institute; Shanghai Key Laboratory of Translational Medicine on Ear 
      and Nose diseases, Shanghai, 200092, China.
FAU - Xu, Dan
AU  - Xu D
AD  - Center for Translational Medicine, Yangpu Hospital, Tongji University School of 
      Medicine, Shanghai, 200031, China.
FAU - Zhang, Yifei
AU  - Zhang Y
AD  - Department of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai 
      Jiaotong University School of Medicine; Shanghai Jiaotong University School of 
      Medicine Ear Institute; Shanghai Key Laboratory of Translational Medicine on Ear 
      and Nose diseases, Shanghai, 200092, China.
FAU - Chen, Penghui
AU  - Chen P
AD  - Department of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai 
      Jiaotong University School of Medicine; Shanghai Jiaotong University School of 
      Medicine Ear Institute; Shanghai Key Laboratory of Translational Medicine on Ear 
      and Nose diseases, Shanghai, 200092, China.
FAU - Xie, Jin
AU  - Xie J
AD  - Department of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai 
      Jiaotong University School of Medicine; Shanghai Jiaotong University School of 
      Medicine Ear Institute; Shanghai Key Laboratory of Translational Medicine on Ear 
      and Nose diseases, Shanghai, 200092, China. xiejin@xinhuamed.com.cn.
LA  - eng
GR  - 81502339/National Natural Science Foundation of China/
GR  - 81402325, 81874151/National Natural Science Foundation of China/
GR  - 20184Y0241/Shanghai Municipal Health and Family Planning Commission/
GR  - 202040162/Shanghai Municipal Health Commission/
PT  - Journal Article
DEP - 20220819
PL  - England
TA  - World J Surg Oncol
JT  - World journal of surgical oncology
JID - 101170544
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptor, Notch1)
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - RNA, Small Interfering/genetics
MH  - *Receptor, Notch1/genetics/metabolism
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/pathology
MH  - Tumor Hypoxia
MH  - Tumor Microenvironment
PMC - PMC9389713
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell hypoxia
OT  - Cell proliferation
OT  - Laryngeal neoplasms
OT  - Notch1
COIS- The authors declare that they have no competing interests.
EDAT- 2022/08/19 06:00
MHDA- 2022/08/23 06:00
PMCR- 2022/08/19
CRDT- 2022/08/18 23:42
PHST- 2022/05/29 00:00 [received]
PHST- 2022/08/09 00:00 [accepted]
PHST- 2022/08/18 23:42 [entrez]
PHST- 2022/08/19 06:00 [pubmed]
PHST- 2022/08/23 06:00 [medline]
PHST- 2022/08/19 00:00 [pmc-release]
AID - 10.1186/s12957-022-02728-6 [pii]
AID - 2728 [pii]
AID - 10.1186/s12957-022-02728-6 [doi]
PST - epublish
SO  - World J Surg Oncol. 2022 Aug 19;20(1):262. doi: 10.1186/s12957-022-02728-6.