PMID- 35986391
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220826
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 53
IP  - 1
DP  - 2022 Aug 19
TI  - A porcine reproductive and respiratory syndrome virus (PRRSV)-specific IgM as a 
      novel adjuvant for an inactivated PRRSV vaccine improves protection efficiency 
      and enhances cell-mediated immunity against heterologous PRRSV challenge.
PG  - 65
LID - 10.1186/s13567-022-01082-5 [doi]
LID - 65
AB  - Current strategies for porcine reproductive and respiratory syndrome (PRRS) 
      control are inadequate and mainly restricted to immunization using different PRRS 
      virus (PPRSV) vaccines. Although there are no safety concerns, the poor 
      performance of inactivated PRRSV vaccines has restricted their practical 
      application. In this research, we employed the novel PRRSV-specific IgM 
      monoclonal antibody (Mab)-PR5nf1 as a vaccine adjuvant for the formulation of a 
      cocktail composed of inactivated PRRSV (KIV) and Mab-PR5nf1 along with a normal 
      adjuvant to enhance PRRSV-KIV vaccine-mediated protection and further compared it 
      with a normal KIV vaccine and modified live virus vaccine (MLV). After challenge 
      with highly pathogenic (HP)-PRRSV, our results suggested that the overall 
      survival rate (OSR) and cell-mediated immunity (CMI), as determined by serum 
      IFN-gamma quantification and IFN-gamma ELISpot assay, were significantly improved by 
      adding PRRSV-specific IgM to the PRRSV-KIV vaccine. It was also notable that both 
      the OSR and CMI in the Mab-PR5nf1-adjuvanted KIV group were even higher than 
      those in the MLV group, whereas the CMI response is normally poorly evoked by KIV 
      vaccines or subunit vaccines. Compared with those in piglets immunized with the 
      normal KIV vaccine, viral shedding and serum neutralizing antibody levels were 
      also improved, and reduced viral shedding appeared to be a result of enhanced CMI 
      caused by the inclusion of IgM as an adjuvant. In conclusion, our data provide 
      not only a new formula for the development of an effective PRRSV-KIV vaccine for 
      practical use but also a novel method for improving antigen-specific CMI 
      induction by inactivated vaccines and subunit vaccines.
CI  - (c) 2022. The Author(s).
FAU - Chen, Rui
AU  - Chen R
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Liu, Bing
AU  - Liu B
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Zhang, Xiangmei
AU  - Zhang X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Qin, Mengmeng
AU  - Qin M
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Dong, Jianhui
AU  - Dong J
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Gu, Guoqian
AU  - Gu G
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
AD  - Department of Infectious Diseases and Public Health, Jockey Club College of 
      Veterinary Medicine, City University of Hong Kong, Hong Kong SAR, China.
FAU - Wu, Chunyan
AU  - Wu C
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Wang, Jingyu
AU  - Wang J
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China. wjingyu2004@126.com.
FAU - Nan, Yuchen
AU  - Nan Y
AUID- ORCID: 0000-0002-4442-8004
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi, China. nanyuchen2015@nwsuaf.edu.cn.
LA  - eng
GR  - 2017YFD0501004/National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20220819
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Antibodies, Viral
MH  - Immunity, Cellular
MH  - Immunoglobulin M
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - *Swine Diseases
MH  - Vaccines, Attenuated
MH  - Vaccines, Inactivated
MH  - Vaccines, Subunit
MH  - *Viral Vaccines
PMC - PMC9389807
OTO - NOTNLM
OT  - IgM
OT  - PRRSV
OT  - broadly neutralizing antibody
OT  - inactivated PRRSV vaccine
OT  - monoclonal antibody
OT  - vaccine adjuvant
COIS- The authors declare that they have no competing interests.
EDAT- 2022/08/20 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/08/19
CRDT- 2022/08/19 23:41
PHST- 2022/04/30 00:00 [received]
PHST- 2022/06/30 00:00 [accepted]
PHST- 2022/08/19 23:41 [entrez]
PHST- 2022/08/20 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/08/19 00:00 [pmc-release]
AID - 10.1186/s13567-022-01082-5 [pii]
AID - 1082 [pii]
AID - 10.1186/s13567-022-01082-5 [doi]
PST - epublish
SO  - Vet Res. 2022 Aug 19;53(1):65. doi: 10.1186/s13567-022-01082-5.