PMID- 35987447
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20221013
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 65
DP  - 2022 Oct
TI  - TM4SF19-AS1 facilitates the proliferation of lung squamous cell carcinoma by 
      recruiting WDR5 to mediate TM4SF19.
PG  - 101849
LID - S0890-8508(22)00060-3 [pii]
LID - 10.1016/j.mcp.2022.101849 [doi]
AB  - BACKGROUND: As reported, long non-coding RNAs are a pivotal player in lung 
      squamous cell carcinoma (LSCC) progression. We noticed the remarkably upregulated 
      transmembrane-4-l-six-family-19 antisense RNA 1 (TM4SF19-AS1) in LSCC and further 
      demonstrated the function it played in LSCC and the possible molecular mechanism. 
      METHODS: Via bioinformatics approach, we evaluated TM4SF19-AS1 and TM4SF19 levels 
      in LSCC tissue, and real-time quantitative polymerase chain reaction (qRT-PCR) 
      and Western blot revealed their mRNA and protein levels in LSCC cells. Cell 
      Counting Kit-8 and colony formation assays analyzed the proliferation ability of 
      LSCC cells, and cell adhesion ability was detected via cell adhesion assay. RNA 
      immunoprecipitation and chromatin immunoprecipitation analyzed the underlying 
      mechanism of TM4SF19-AS1 regulating its target, while methylation-specific PCR 
      indicated the methylation level of TM4SF19-AS1. RESULTS: TM4SF19-AS1 was markedly 
      upregulated in LSCC. Functional assays revealed that TM4SF19-AS1 could facilitate 
      the proliferation and adhesion of LSCC. Besides, we revealed the mechanism of 
      TM4SF19-AS1 regulation that it directly bound to WD repeat-containing protein 5 
      (WDR5), and was then recruited to TM4SF19 promoter region, which activated DNA 
      demethylation, thereby suppressing malignant LSCC progression. CONCLUSION: Our 
      research demonstrated that TM4SF19-AS1 affected LSCC cell proliferation by 
      recruiting WDR5 to manipulate transmembrane-4-lsix-family-member-19 (TM4SF19), 
      which offers a new observation on LSCC pathogenesis, indicating that TM4SF19-AS1 
      is able to be a promising target for LSCC treatment.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Luo, Machang
AU  - Luo M
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Xie, Lingyan
AU  - Xie L
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Su, Yonghua
AU  - Su Y
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Zhang, Kaijun
AU  - Zhang K
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Liang, Rongzhang
AU  - Liang R
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Ma, Zhiyi
AU  - Ma Z
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China.
FAU - Li, Youtang
AU  - Li Y
AD  - Department of Respiratory and Critical Care Medicine, The First Hospital of 
      Longyan Affiliated to Fujian Medical University, Longyan City, Fujian Province, 
      364000, China. Electronic address: lyongt1@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220818
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Tetraspanins)
RN  - 0 (WDR5 protein, human)
SB  - IM
MH  - *Carcinoma, Non-Small-Cell Lung/genetics
MH  - *Carcinoma, Squamous Cell/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Lung/metabolism
MH  - *Lung Neoplasms/genetics
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Tetraspanins
OTO - NOTNLM
OT  - Adhesion
OT  - Lung squamous cell carcinoma
OT  - Proliferation
OT  - TM4SF19
OT  - TM4SF19-AS1
OT  - WDR5
COIS- Declaration of competing interest The authors report no conflict of interest.
EDAT- 2022/08/21 06:00
MHDA- 2022/09/09 06:00
CRDT- 2022/08/20 19:34
PHST- 2022/06/13 00:00 [received]
PHST- 2022/08/02 00:00 [revised]
PHST- 2022/08/03 00:00 [accepted]
PHST- 2022/08/21 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/08/20 19:34 [entrez]
AID - S0890-8508(22)00060-3 [pii]
AID - 10.1016/j.mcp.2022.101849 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2022 Oct;65:101849. doi: 10.1016/j.mcp.2022.101849. Epub 2022 
      Aug 18.