PMID- 35988282
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220901
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 68
IP  - 4
DP  - 2022 Apr 30
TI  - Effect of Rosuvastatin on Myocardial Apoptosis in HypertensiveRats Through 
      SIRT1/NF-kappaB Signaling Pathway.
PG  - 194-201
LID - 10.14715/cmb/2022.68.4.23 [doi]
AB  - The study aimed to observe the effect of rosuvastatin on myocardial apoptosis in 
      hypertensive rats through the silent information regulator 1 (SIRT1)/nuclear 
      factor-kappaB (NF-kappaB) signaling pathway. The spontaneously hypertensive rat (SHR) 
      model was established, and the rats were randomly divided into the SHR group, 
      Rosuvastatin group and Control group. The blood pressure, creatine kinase (CK) 
      and other myocardial indexes in each group were detected, the cardiac function 
      indexes of rats were determined using magnetic resonance imaging (MRI) and 
      echocardiography (ECG), and tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 
      (IL-6) in myocardial tissues were detected via enzyme-linked immunosorbent assay 
      (ELISA). Moreover, the apoptosis level of myocardial tissues was detected using 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      staining. Finally, the expression levels of the SIRT1/NF-kappaB signaling pathway and 
      apoptosis genes and proteins in myocardial tissues in each group were detected 
      via quantitative polymerase chain reaction (qPCR) and Western blotting. In the 
      SHR group, the blood pressure, the levels of serum creatinine (CR) and CK were 
      increased (p<0.05). In the SHR group, both fractional shortening (FS%) and 
      ejection fraction (EF%) were obviously lower than those in the control group 
      (p<0.05), while both left ventricular end-diastolic diameter (LVEDd) and left 
      ventricular end-systolic diameter (LVESd) were higher than those in the control 
      group (p<0.05), and the levels of TNF-alpha, IL-6 and myeloperoxidase (MPO) were 
      increased (p<0.05). The number of apoptotic cells in myocardial tissues in the 
      SHR group was larger than that in the other two groups (p<0.05). In the SHR 
      group, the expression levels of Caspase3 and NF-kappaB were remarkably higher than 
      those in the Rosuvastatin group (p<0.05), while the expression levels of Bcl-2 
      and SIRT1 were remarkably lower than those in the Rosuvastatin group (p<0.05). 
      Rosuvastatin can inhibit myocardial apoptosis in hypertensive rats through 
      up-regulating SIRT1 and down-regulating NF-kappaB.
FAU - Ren, Xiangqian
AU  - Ren X
AD  - Department of Cardiology, Anning Branch, 940 Hospital of the Joint Service 
      support Force of the Chinese People's Liberation Army, Gansu, 730070, China. 
      yuhuicuo35838@163.com.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Cardiology, Anning Branch, 940 Hospital of the Joint Service 
      support Force of the Chinese People's Liberation Army, Gansu, 730070, China. 
      yuhuicuo35838@163.com.
FAU - Han, Lin
AU  - Han L
AD  - Department of Cardiology, Anning Branch, 940 Hospital of the Joint Service 
      support Force of the Chinese People's Liberation Army, Gansu, 730070, China. 
      yuhuicuo35838@163.com.
FAU - Sun, Zhenzhen
AU  - Sun Z
AD  - Department of Cardiology, Anning Branch, 940 Hospital of the Joint Service 
      support Force of the Chinese People's Liberation Army, Gansu, 730070, China. 
      yuhuicuo35838@163.com.
FAU - Yuan, Bin
AU  - Yuan B
AD  - Department of Cardiology, Anning Branch, 940 Hospital of the Joint Service 
      support Force of the Chinese People's Liberation Army, Gansu, 730070, China. 
      yuhuicuo35838@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220430
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83MVU38M7Q (Rosuvastatin Calcium)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Disease Models, Animal
MH  - Interleukin-6/pharmacology
MH  - Myocardium/metabolism
MH  - *NF-kappa B/metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - *Rosuvastatin Calcium/pharmacology/therapeutic use
MH  - Signal Transduction
MH  - *Sirtuin 1/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2022/08/22 06:00
MHDA- 2022/08/24 06:00
CRDT- 2022/08/21 16:16
PHST- 2022/04/25 00:00 [received]
PHST- 2022/08/21 16:16 [entrez]
PHST- 2022/08/22 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
AID - 10.14715/cmb/2022.68.4.23 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2022 Apr 30;68(4):194-201. doi: 
      10.14715/cmb/2022.68.4.23.