PMID- 35988608
OWN - NLM
STAT- MEDLINE
DCOM- 20221019
LR  - 20221019
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 851
IP  - Pt 1
DP  - 2022 Dec 10
TI  - Influence of lactate to acetate ratio on biological production of medium chain 
      carboxylates via open culture fermentation.
PG  - 158171
LID - S0048-9697(22)05270-6 [pii]
LID - 10.1016/j.scitotenv.2022.158171 [doi]
AB  - Waste valorisation via biological production of widely used in the industry 
      medium chain carboxylates (MCCs) via open culture fermentation (OCF) could be a 
      promising alternative to the commonly used anaerobic digestion. Lactate-rich 
      waste streams are considered as valuable substrates for carboxylate chain 
      elongation (CE), however, there are certain limitations related to the production 
      efficiency. Acetate produced and accumulated in the acetogenesis plays an 
      important role in CE, i.e. acetate is elongated to butyrate and then to caproate 
      which is most popular MCC. Henceforth, it was investigated whether the ratio of 
      lactate to acetate (L:A) affected carboxylates yields and product distribution in 
      the lactate-based CE in OCF. The tested L:A ratios influenced carboxylates 
      selectivity in batch trials. In the ones with lactate as the sole carbon source, 
      propionate production was predominant but when a higher relative acetate 
      concentration was used, the production of butyrate and CE to caproate was 
      favored. The co-utilization of lactate and acetate in a continuous process 
      increased the production of butyrate and caproate compared to the phase with 
      lactate as the sole carbon source, however, controlling the relative 
      concentration of lactate and acetate during co-utilization was not an effective 
      strategy for increasing caproate production. 16S rRNA gene amplicon reads mapping 
      to Caproiciproducens were the most abundant in samples collected throughout the 
      continuous processes regardless of the L:A ratios.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Brodowski, Filip
AU  - Brodowski F
AD  - Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and 
      Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
FAU - Lezyk, Mateusz
AU  - Lezyk M
AD  - Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and 
      Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
FAU - Gutowska, Natalia
AU  - Gutowska N
AD  - Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and 
      Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
FAU - Kabasakal, Tugba
AU  - Kabasakal T
AD  - Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and 
      Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
FAU - Oleskowicz-Popiel, Piotr
AU  - Oleskowicz-Popiel P
AD  - Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and 
      Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland. 
      Electronic address: piotr.oleskowicz-popiel@put.poznan.pl.
LA  - eng
PT  - Journal Article
DEP - 20220818
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Acetates)
RN  - 0 (Butyrates)
RN  - 0 (Caproates)
RN  - 0 (Propionates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 1F8SN134MX (hexanoic acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Acetates
MH  - Butyrates
MH  - *Caproates
MH  - Carbon
MH  - Fermentation
MH  - *Lactic Acid
MH  - Propionates
MH  - RNA, Ribosomal, 16S
OTO - NOTNLM
OT  - Acetate
OT  - Caproate
OT  - Caproiciproducens
OT  - Chain elongation
OT  - Lactate
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/08/22 06:00
MHDA- 2022/10/20 06:00
CRDT- 2022/08/21 19:23
PHST- 2022/07/01 00:00 [received]
PHST- 2022/08/08 00:00 [revised]
PHST- 2022/08/16 00:00 [accepted]
PHST- 2022/08/22 06:00 [pubmed]
PHST- 2022/10/20 06:00 [medline]
PHST- 2022/08/21 19:23 [entrez]
AID - S0048-9697(22)05270-6 [pii]
AID - 10.1016/j.scitotenv.2022.158171 [doi]
PST - ppublish
SO  - Sci Total Environ. 2022 Dec 10;851(Pt 1):158171. doi: 
      10.1016/j.scitotenv.2022.158171. Epub 2022 Aug 18.