PMID- 35989741
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220823
IS  - 2168-8184 (Print)
IS  - 2168-8184 (Electronic)
IS  - 2168-8184 (Linking)
VI  - 14
IP  - 7
DP  - 2022 Jul
TI  - The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice.
PG  - e26903
LID - 10.7759/cureus.26903 [doi]
LID - e26903
AB  - Objective  Through the preparation of the diabetic mice skin ulcer model, we 
      investigated the effect of Mongolian medicine external ulcer powder (WYK) on the 
      treatment of diabetic skin ulcers and the expression of angiogenesis-related 
      factors such as vascular endothelial growth factor (VEGF) and extracellular 
      regulated protein kinases (ERK). Methods  Thirty male clean Kunming mice were 
      randomly divided into normal control group (group C), diabetic control group 
      (group HC), and diabetic topical ulcer powder group (group HW). After successful 
      modeling in the HC group and the HW group, the rats in the HW group were given 
      external ulcer powder, which was applied to the back of the mice once a day. In 
      addition, the rats in group C and group HC were treated with gentamicin injection 
      external application once a day. The mice were sacrificed on the 3rd, the sixth, 
      and the ninth day of dosing, and samples were taken. The adopted methods included 
      protein immunoblotting (western blot) and reverse transcription-polymerase chain 
      reaction (RT-PCR). The expression differences of angiogenesis-related factors 
      such as VEGF and ERK in the repair process were detected. SPSS 13 software was 
      used to analyze the results of angiogenesis-related factors VEGF and ERK. Results 
      Comparison of VEGF and ERK Contents The serum VEGF content of mice in the HC 
      group was significantly lower than that in the C group on days 3, 6, and 9 (p 
      <0.05). The VEGF content in the HW group was significantly higher than that in 
      the HC group (p <0.05). The content of ERK in serum was basically consistent with 
      that of VEGF. The results of the western blot assay were consistent with those of 
      the RT-PCR assay. Conclusion  WYK can effectively promote the healing of skin 
      ulcer wounds in diabetic mice, accelerate the proliferation of granulation 
      tissue, enrich the contents of capillary blood tubes and collagen fibers, and 
      increase the microvascular content. WYK can improve the expression level of VEGF 
      and ERK in the serum of mice and advance the peak value of protein expression.
CI  - Copyright (c) 2022, Wang et al.
FAU - Wang, Bingyang
AU  - Wang B
AD  - Department of Pathology, Seventh Affiliated Hospital, Sun Yat-sen University, 
      Shenzhen, CHN.
FAU - Xie, Wenlin
AU  - Xie W
AD  - Department of Pathology, Seventh Affiliated Hospital, Sun Yat-sen University, 
      Shenzhen, CHN.
FAU - Lu, Xiaofang
AU  - Lu X
AD  - Department of Pathology, Seventh Affiliated Hospital, Sun Yat-sen University, 
      Shenzhen, CHN.
FAU - Song, Jian
AU  - Song J
AD  - Department of Pathology, Seventh Affiliated Hospital, Sun Yat-sen University, 
      Shenzhen, CHN.
FAU - Liang, Hongsen
AU  - Liang H
AD  - Department of Thoracic Surgery, Seventh Affiliated Hospital, Sun Yat-sen 
      University, Shenzhen, CHN.
LA  - eng
PT  - Journal Article
DEP - 20220715
PL  - United States
TA  - Cureus
JT  - Cureus
JID - 101596737
PMC - PMC9378942
OTO - NOTNLM
OT  - diabetes foot ulcer
OT  - effect
OT  - mice
OT  - ulcer
OT  - wyk
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/23 06:01
PMCR- 2022/07/15
CRDT- 2022/08/22 03:45
PHST- 2022/07/15 00:00 [accepted]
PHST- 2022/08/22 03:45 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/23 06:01 [medline]
PHST- 2022/07/15 00:00 [pmc-release]
AID - 10.7759/cureus.26903 [doi]
PST - epublish
SO  - Cureus. 2022 Jul 15;14(7):e26903. doi: 10.7759/cureus.26903. eCollection 2022 
      Jul.