PMID- 35990247
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220823
IS  - 1875-8630 (Electronic)
IS  - 0278-0240 (Print)
IS  - 0278-0240 (Linking)
VI  - 2022
DP  - 2022
TI  - The Role of Gap Junctions in the Generation of Smooth Muscle Cells from Bone 
      Marrow Mesenchymal Stem Cells.
PG  - 1491327
LID - 10.1155/2022/1491327 [doi]
LID - 1491327
AB  - BACKGROUND: Studies have shown that stem cell transplantation can improve smooth 
      muscle cell (SMC) regeneration and remodelling. Gap junctions can enhance the 
      cytoprotective effects of neighbouring cells. We investigated the effect of gap 
      junctions on the differentiation of bone marrow mesenchymal stem cells (BMSCs) 
      into SMCs. MATERIALS AND METHODS: Rat BMSCs and SMCs were obtained from the bone 
      marrow and bladder of Sprague-Dawley rats, respectively. Flow cytometry and 
      multilineage differentiation were performed to assess the characteristics of 
      these cells. BMSCs and SMCs were incubated together in cocultures in the presence 
      and absence of heptanol, an uncoupler of gap junctions. Cocultures were divided 
      into three groups consisting of a contact coculture, noncontact coculture, and 
      contact coculture plus heptanol groups. The expression of BMSC-specific markers 
      and the effect of gap junctions on the differentiation of BMSCs were evaluated by 
      performing real-time reverse transcription-polymerase chain reaction, 
      immunofluorescence analysis, and western blotting after cocultures. RESULTS: CD90 
      and CD44 were markedly expressed, and CD31 and CD45 were weakly or not expressed 
      in BMSCs. The cells also showed good osteogenic and adipogenic differentiation 
      ability. Compared with the noncontact coculture group, the SMC markers such as 
      alpha-SMA, calponin, and connexin43 increased in the contact coculture group. The 
      effect of contact in the coculture group was significantly weakened by heptanol. 
      CONCLUSIONS: The results suggested that gap junctions play an important role in 
      the generation of SMCs from BMSCs. The formation of SMCs can potentially be used 
      to repair the sphincter muscle of patients with stress urinary incontinence.
CI  - Copyright (c) 2022 Ying Wang et al.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Yang, Boping
AU  - Yang B
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Hu, Pan
AU  - Hu P
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Lu, Shentao
AU  - Lu S
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Lei, Li
AU  - Lei L
AUID- ORCID: 0000-0003-2806-210X
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Liu, Lubin
AU  - Liu L
AUID- ORCID: 0000-0002-5718-2541
AD  - Department of Obstetrics and Gynecology, Chongqing Health Center for Women and 
      Children, Women and Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20220812
PL  - United States
TA  - Dis Markers
JT  - Disease markers
JID - 8604127
RN  - 8JQ5607IO5 (Heptanol)
SB  - IM
MH  - Animals
MH  - *Bone Marrow Cells/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Gap Junctions
MH  - Heptanol/metabolism/pharmacology
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC9391152
COIS- The authors report no conflicts of interest in this work.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/08/12
CRDT- 2022/08/22 03:52
PHST- 2022/06/07 00:00 [received]
PHST- 2022/08/02 00:00 [revised]
PHST- 2022/08/03 00:00 [accepted]
PHST- 2022/08/22 03:52 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/08/12 00:00 [pmc-release]
AID - 10.1155/2022/1491327 [doi]
PST - epublish
SO  - Dis Markers. 2022 Aug 12;2022:1491327. doi: 10.1155/2022/1491327. eCollection 
      2022.