PMID- 35990638
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20221207
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Dietary supplementation of exopolysaccharides from Lactobacillus rhamnosus GCC-3 
      improved the resistance of zebrafish against spring viremia of carp virus 
      infection.
PG  - 968348
LID - 10.3389/fimmu.2022.968348 [doi]
LID - 968348
AB  - Spring viremia of carp virus (SVCV) can cause high mortality of fish. The aim of 
      this study was to investigate the effects of Lactobacillus rhamnosus GCC-3 
      exopolysaccharides (GCC-3 EPS) on zebrafish (Danio rerio) infected with SVCV and 
      elucidate the underlying mechanisms. Zebrafish were fed with a control diet or 
      diet supplemented with 0.5% and 1% of GCC-3 EPS for 2 weeks. The results showed 
      that supplementation of GCC-3 EPS significantly improved the survival rate of 
      zebrafish compared with the control group. In addition, dietary 0.5% and 1% GCC-3 
      EPS significantly up-regulated the expression of genes related to type I 
      interferon (IFN) antiviral immunity. Consistent with in vivo results, GCC-3 EPS 
      significantly inhibited SVCV replication in zebrafish embryonic fibroblast (ZF4) 
      cells while significantly increased the expression of type I IFN signaling 
      pathway related genes. Furthermore, knocking down TANK-binding kinase 1 
      significantly blocked the antiviral effect of GCC-3 EPS. Dietary GCC-3 EPS 
      improved gut microbiota, and the culture supernatant of GCC-3 EPS-associated 
      microbiota significantly inhibited SVCV replication in ZF4 cells compared with 
      the control-microbiota counterpart. In conclusion, our results indicate that 
      dietary GCC-3 EPS can improve the resistance of zebrafish against SVCV infection, 
      and the mechanism may involve enhanced type I interferon signaling.
CI  - Copyright (c) 2022 Xie, Li, Olsen, Ringo, Yang, Zhang, Ran and Zhou.
FAU - Xie, Mingxu
AU  - Xie M
AD  - Sino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese 
      Academy of Agricultural Sciences, Beijing, China.
AD  - Norway-China Joint Lab on Fish Gastrointestinal Microbiota, Institute of Biology, 
      Norwegian University of Science and Technology, Trondheim, Norway.
FAU - Li, Yu
AU  - Li Y
AD  - Sino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese 
      Academy of Agricultural Sciences, Beijing, China.
AD  - Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, 
      Shaanxi Normal University, Xi'an, China.
FAU - Olsen, Rolf Erik
AU  - Olsen RE
AD  - Norway-China Joint Lab on Fish Gastrointestinal Microbiota, Institute of Biology, 
      Norwegian University of Science and Technology, Trondheim, Norway.
FAU - Ringo, Einar
AU  - Ringo E
AD  - Norwegian College of Fisheries Science, Faculty of Biosciences, Fisheries and 
      Economics, University of Tromso (UiT) The Arctic University of Norway, Tromso, 
      Norway.
FAU - Yang, Yalin
AU  - Yang Y
AD  - Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural 
      Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 
      Beijing, China.
FAU - Zhang, Zhen
AU  - Zhang Z
AD  - Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural 
      Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 
      Beijing, China.
FAU - Ran, Chao
AU  - Ran C
AD  - Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural 
      Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 
      Beijing, China.
FAU - Zhou, Zhigang
AU  - Zhou Z
AD  - Sino-Norway Joint Lab on Fish Gut Microbiota, Institute of Feed Research, Chinese 
      Academy of Agricultural Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220805
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antiviral Agents)
RN  - 0 (Interferon Type I)
RN  - Carp sprivivirus
SB  - IM
MH  - Animals
MH  - Antiviral Agents/therapeutic use
MH  - *Carps
MH  - Dietary Supplements
MH  - *Fish Diseases
MH  - *Interferon Type I/therapeutic use
MH  - *Lacticaseibacillus rhamnosus
MH  - Rhabdoviridae
MH  - *Rhabdoviridae Infections/veterinary
MH  - Viremia
MH  - Zebrafish
PMC - PMC9389081
OTO - NOTNLM
OT  - GCC-3 EPS
OT  - gut microbiota
OT  - spring viremia of carp virus
OT  - type I IFN
OT  - zebrafish
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/01/01
CRDT- 2022/08/22 03:58
PHST- 2022/06/15 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/08/22 03:58 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.968348 [doi]
PST - epublish
SO  - Front Immunol. 2022 Aug 5;13:968348. doi: 10.3389/fimmu.2022.968348. eCollection 
      2022.