PMID- 35990904
OWN - NLM
STAT- MEDLINE
DCOM- 20230117
LR  - 20230117
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 10
DP  - 2022
TI  - Integrated bioinformatics analysis identifies the effects of Sema3A/NRP1 
      signaling in oligodendrocytes after spinal cord injury in rats.
PG  - e13856
LID - 10.7717/peerj.13856 [doi]
LID - e13856
AB  - OBJECTIVE: To investigate the effect of Sema3A/NRP1 signaling in oligodendrocytes 
      (OLs) after spinal cord injury. METHODS: Three analysis strategies, namely 
      differential expression gene analysis, Gene Ontology (GO) enrichment, and Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) pathway analysis, were applied. The 
      protein-protein interaction (PPI) network was constructed using the STRING 
      website to explore the correlation between Sema3A/NRP1 and oligodendrocytes. 
      Then, the T10 spinal cord segment of rats was injured by the Allen method to 
      establish a spinal cord injury (SCI) model. Real-time quantitative PCR, Western 
      blotting, Nissl staining and immunofluorescence staining were used to detect the 
      effect of Sema3A/NRP1 signaling on oligodendrocytes in vivo. RESULTS: After the 
      SCI model was established, significantly fewer oligodendrocytes were observed. At 
      the same time, R software was used to analyze the expression of related genes, 
      and NRP1 expression was increased. PCR also demonstrated similar results, and 
      NRP1 ligand Sema3A was also upregulated. KEGG and GO functional enrichment 
      analysis indicated that the SCI model was mainly related to cytokine interaction, 
      cell proliferation, differentiation and maturation. Interestingly, we found that 
      NRP1 was involved in semaphorin-plexin signaling pathway neuronal projection 
      guidance and axon guidance, mediating cell growth and migration. Moreover, 
      Sema3A/NRP1 signaling was closely associated with platelet-derived growth factor 
      receptor alpha (PDGFRalpha) in the PPI network. When Sema3A/NRP1 signaling was 
      specifically blocked at early stages, PDGFRalpha expression was effectively 
      inhibited, and the expression of OLs was promoted. Furthermore, inhibition of 
      Sema3A/NRP1 signaling increased the Basso-Beattie-Bresnahan (BBB) score of lower 
      limb motor function in SCI rats and promoted the survival of motor neurons in the 
      ventral horn of the injured spinal cord. CONCLUSION: Our data suggest that 
      Sema3A/NRP1 signaling may regulate the development of OPCs and OLs after SCI, 
      thereby affecting functional recovery.
CI  - (c)2022 Hu et al.
FAU - Hu, Rong
AU  - Hu R
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
FAU - Shi, Mengting
AU  - Shi M
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
FAU - Xu, Haipeng
AU  - Xu H
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
FAU - Wu, Xingying
AU  - Wu X
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
FAU - He, Kelin
AU  - He K
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
AD  - Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of 
      Zhejiang Chinese Medical University (Zhongshan Hospital of Zhejiang Province), 
      HangZhou, China.
FAU - Chen, Yi
AU  - Chen Y
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
FAU - Wu, Lei
AU  - Wu L
AD  - Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of 
      Zhejiang Chinese Medical University (Zhongshan Hospital of Zhejiang Province), 
      HangZhou, China.
FAU - Ma, Ruijie
AU  - Ma R
AD  - Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology 
      of Zhejiang Province, Third School of Clinical Medicine (School of Rehabilitation 
      Medicine), HangZhou, China.
AD  - Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of 
      Zhejiang Chinese Medical University (Zhongshan Hospital of Zhejiang Province), 
      HangZhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220816
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
RN  - 0 (Semaphorin-3A)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
MH  - Rats
MH  - Animals
MH  - *Semaphorin-3A/genetics
MH  - Receptor, Platelet-Derived Growth Factor alpha/metabolism
MH  - *Spinal Cord Injuries/genetics
MH  - Signal Transduction/genetics
MH  - Oligodendroglia/metabolism
MH  - Computational Biology
PMC - PMC9390322
OTO - NOTNLM
OT  - Bioinformatic analysis
OT  - Oligodendrocyte
OT  - PDGFRalpha
OT  - Sema3A/NRP1signal
OT  - Spinal cord injury
COIS- The authors declare there are no competing interests.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/23 06:01
PMCR- 2022/08/16
CRDT- 2022/08/22 04:03
PHST- 2022/03/10 00:00 [received]
PHST- 2022/07/16 00:00 [accepted]
PHST- 2022/08/22 04:03 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/23 06:01 [medline]
PHST- 2022/08/16 00:00 [pmc-release]
AID - 13856 [pii]
AID - 10.7717/peerj.13856 [doi]
PST - epublish
SO  - PeerJ. 2022 Aug 16;10:e13856. doi: 10.7717/peerj.13856. eCollection 2022.