PMID- 35991985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220823
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 8
IP  - 8
DP  - 2022 Aug
TI  - Evaluation of anthoxanthins and their actions on digestive enzyme inhibition when 
      used independently and in combination.
PG  - e10131
LID - 10.1016/j.heliyon.2022.e10131 [doi]
LID - e10131
AB  - Carbohydrate digestibility is a key determinant for elevated postprandial 
      hyperglycemia (PPHG). Apart from dietary restrictions, one of the strategies to 
      reduce PPHG is to limit the activity of carbohydrate digestive enzymes within the 
      gastrointestinal tract in order to reduce monosaccharide absorption rates. The 
      present work aimed to assess the inhibitory capabilities of digestive enzymes 
      (e.g., alpha-glucosidase and alpha-amylase) by anthoxanthins when used independently, in 
      combination with acarbose, or with a different anthoxanthin. Our results showed 
      that quercetin, myricetin, and luteolin presented lower IC(50) values than 
      acarbose and inhibited alpha-glucosidase through mixed-type inhibition. On the other 
      hand, acarbose when compared with these anthoxanthins, remained the most potent 
      inhibitor of alpha-amylase. Combinatorial treatment (i) acarbose-quercetin and (ii) 
      myricetin-luteolin showed synergistic activity (CI value less than 0.9) in 
      alpha-glucosidase inhibition. An additive effect (CI value between 0.9 and 1.1) in 
      alpha-glucosidase inhibition was observed when acarbose-myricetin, acarbose-luteolin 
      or when a combination of two different anthoxanthins (quercetin-myricetin and 
      quercetin-luteolin) was used. This study suggests the potential use of 
      anthoxanthins as functional food ingredients to mitigate PPHG towards the 
      management of T2DM.
CI  - (c) 2022 The Author(s).
FAU - Koh, Yong Qin
AU  - Koh YQ
AD  - Department of Pharmacy, Faculty of Science, National University of Singapore, 
      Singapore.
FAU - Sin, Yu Ang Desmond
AU  - Sin YAD
AD  - Department of Pharmacy, Faculty of Science, National University of Singapore, 
      Singapore.
FAU - Rong, Hengyang Justin
AU  - Rong HJ
AD  - Department of Pharmacy, Faculty of Science, National University of Singapore, 
      Singapore.
FAU - Chua, Teng Hui Sean
AU  - Chua THS
AD  - Department of Pharmacy, Faculty of Science, National University of Singapore, 
      Singapore.
FAU - Ho, Si-Han Sherman
AU  - Ho SS
AD  - Hoow Foods Pte Ltd, 67 Ayer Rajah Crescent, Singapore.
FAU - Ho, Han Kiat
AU  - Ho HK
AD  - Department of Pharmacy, Faculty of Science, National University of Singapore, 
      Singapore.
LA  - eng
PT  - Journal Article
DEP - 20220808
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9389255
OTO - NOTNLM
OT  - Acarbose
OT  - Anthoxanthins
OT  - Synergistic inhibition
OT  - alpha-amylase
OT  - alpha-glucosidase
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/23 06:01
PMCR- 2022/08/08
CRDT- 2022/08/22 04:20
PHST- 2022/04/24 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/07/27 00:00 [accepted]
PHST- 2022/08/22 04:20 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/23 06:01 [medline]
PHST- 2022/08/08 00:00 [pmc-release]
AID - S2405-8440(22)01419-0 [pii]
AID - e10131 [pii]
AID - 10.1016/j.heliyon.2022.e10131 [doi]
PST - epublish
SO  - Heliyon. 2022 Aug 8;8(8):e10131. doi: 10.1016/j.heliyon.2022.e10131. eCollection 
      2022 Aug.