PMID- 35992546
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20221006
IS  - 1555-4317 (Electronic)
IS  - 1555-4309 (Print)
IS  - 1555-4309 (Linking)
VI  - 2022
DP  - 2022
TI  - Circular RNA MELK Promotes Chondrocyte Apoptosis and Inhibits Autophagy in 
      Osteoarthritis by Regulating MYD88/NF-kappaB Signaling Axis through MicroRNA-497-5p.
PG  - 7614497
LID - 10.1155/2022/7614497 [doi]
LID - 7614497
AB  - Osteoarthritis (OA) is a rheumatic disease and its pathogenesis involves the 
      dysregulation of noncoding RNAs. Therefore, the regulatory mechanism of circular 
      RNA MELK (circMELK) was specified in this work. OA human cartilage tissue was 
      collected, and circMELK, miR-497-5p, and myeloid differentiation factor 88 
      (MYD88) expression were examined. Human chondrocytes were stimulated with 
      interleukin- (IL-) 1beta and interfered with vectors altering circMELK, miR-497-5p, 
      and MyD88 expression to observe their effects on cell viability, cell cycle and 
      apoptosis, autophagy, and inflammation. The binding relationship between RNAs was 
      verified. The data presented that OA cartilage tissues presented raised circMELK 
      and MYD88 and inhibited miR-497-5p expression. IL-1beta suppressed cell viability, 
      prevented cell cycle, and induced apoptosis, autophagy, and inflammation of 
      chondrocytes. Functionally, IL-1beta-induced changes of chondrocytes could be 
      attenuated by suppressing circMELK or overexpressing miR-497-5p. circMELK acted 
      as a sponge of miR-497-5p while miR-497-5p was a regulator of MYD88. MYD88 
      restricted the effect of overexpressing miR-497-5p on IL-1beta-stimulated 
      chondrocytes. MYD88 triggered nuclear factor-kappaB (NF-kappaB) pathway activation. 
      Shortly, CircMELK promotes chondrocyte apoptosis and inhibits autophagy in OA by 
      regulating MYD88/NF-kappaB signaling axis through miR-497-5p. Our study proposes a 
      new molecular mechanism for the development of OA.
CI  - Copyright (c) 2022 Yingchi Zhang et al.
FAU - Zhang, Yingchi
AU  - Zhang Y
AUID- ORCID: 0000-0002-3892-2946
AD  - Department of Traumatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Lu, Rui
AU  - Lu R
AUID- ORCID: 0000-0002-4909-4007
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Huang, Xiaojian
AU  - Huang X
AUID- ORCID: 0000-0003-0047-6343
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Yin, Enzhi
AU  - Yin E
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Yang, Yong
AU  - Yang Y
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Yi, Chengla
AU  - Yi C
AUID- ORCID: 0000-0003-2585-3571
AD  - Department of Traumatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - You, Hongbo
AU  - You H
AUID- ORCID: 0000-0001-6284-643X
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Song, Xianzhou
AU  - Song X
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
FAU - Yuan, Xuefeng
AU  - Yuan X
AUID- ORCID: 0000-0002-6620-7528
AD  - Department of Traumatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430030, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220730
PL  - England
TA  - Contrast Media Mol Imaging
JT  - Contrast media & molecular imaging
JID - 101286760
RN  - 0 (MIRN497 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Circular)
RN  - EC 2.7.1.- (MELK protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis/genetics/physiology
MH  - Autophagy/genetics/physiology
MH  - Chondrocytes/metabolism
MH  - Humans
MH  - Inflammation/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Myeloid Differentiation Factor 88/genetics/metabolism
MH  - *NF-kappa B/genetics/metabolism
MH  - *Osteoarthritis/genetics/metabolism/pathology
MH  - *Protein Serine-Threonine Kinases/genetics/metabolism
MH  - *RNA, Circular/genetics/metabolism
PMC - PMC9356867
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/07/30
CRDT- 2022/08/22 04:29
PHST- 2022/05/09 00:00 [received]
PHST- 2022/06/09 00:00 [revised]
PHST- 2022/06/15 00:00 [accepted]
PHST- 2022/08/22 04:29 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/07/30 00:00 [pmc-release]
AID - 10.1155/2022/7614497 [doi]
PST - epublish
SO  - Contrast Media Mol Imaging. 2022 Jul 30;2022:7614497. doi: 10.1155/2022/7614497. 
      eCollection 2022.