PMID- 35992691
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220823
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - A microsatellite DNA-derived oligodeoxynucleotide attenuates 
      lipopolysaccharide-induced acute lung injury in mice by inhibiting the 
      HMGB1-TLR4-NF-kappaB signaling pathway.
PG  - 964112
LID - 10.3389/fmicb.2022.964112 [doi]
LID - 964112
AB  - Acute lung injury (ALI) with uncontrolled inflammatory response has high 
      morbidity and mortality rates in critically ill patients. Pathogen-associated 
      molecular patterns (PAMPs) are involved in the development of uncontrolled 
      inflammatory response injury and associated lethality. In this study, we 
      investigated the inhibit effect of MS19, a microsatellite DNA-derived 
      oligodeoxynucleotide (ODN) with AAAG repeats, on the inflammatory response 
      induced by various PAMPs in vitro and in vivo. In parallel, a microsatellite DNA 
      with AAAC repeats, named as MS19-C, was used as controls. We found that MS19 
      extensively inhibited the expression of inflammatory cytokines interleukin (IL)-6 
      and tumor necrosis factor (TNF)-alpha induced by various PAMPs stimulation, including 
      DNA viruses, RNA viruses, bacterial components lipopolysaccharide (LPS), and 
      curdlan, as well as the dsDNA and dsRNA mimics, in primed bone marrow-derived 
      macrophage (BMDM). Other than various PAMPs, MS19 also demonstrated obvious 
      effects on blocking the high mobility group box1 (HMGB1), a representative 
      damage-associated-molecular pattern (DAMP), nuclear translocation and secretion. 
      With the base substitution from G to C, MS19-C has been proved that it has lost 
      the inhibitory effect. The inhibition is associated with nuclear factor kappa B 
      (NF-kappaB) signaling but not the mitogen-activated protein kinase (MAPK) 
      transduction. Moreover, MS19 capable of inhibiting the IL-6 and TNF-alpha production 
      and blocking the HMGB1 nuclear translocation and secretion in LPS-stimulated 
      cells was used to treat mice ALI induced by LPS in vivo. In the ALI mice model, 
      MS19 significantly inhibited the weight loss and displayed the dramatic effect on 
      lessening the ALI by reducing consolidation, hemorrhage, intra-alveolar edema in 
      lungs of the mice. Meanwhile, MS19 could increase the survival rate of ALI by 
      downregulating the inflammation cytokines HMGB1, TNF-a, and IL-6 production in 
      the bronchoalveolar lavage fluid (BALF). The data suggest that MS19 might display 
      its therapeutic role on ALI by inhibiting the HMGB1-TLR4-NF-kappaB signaling pathway.
CI  - Copyright (c) 2022 Zhang, Wang, Wang, Shi, Zhao, Su, Wang, Yang and Fang.
FAU - Zhang, Chenghua
AU  - Zhang C
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
AD  - Department of Endoscopy, Jilin Provincial Cancer Hospital, Changchun, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Wang, Hongrui
AU  - Wang H
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Shi, Shuyou
AU  - Shi S
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Zhao, Peiyan
AU  - Zhao P
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Su, Yingying
AU  - Su Y
AD  - Department of Anatomy, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
FAU - Wang, Hua
AU  - Wang H
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Yang, Ming
AU  - Yang M
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Fang, Mingli
AU  - Fang M
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20220804
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9386506
OTO - NOTNLM
OT  - NF-kappaB - nuclear factor kappa B
OT  - acute lung injury (ALI)
OT  - high-mobility group box 1 (HMGB1)
OT  - inflammation
OT  - oligodeoxynucleotide (ODN)
OT  - pathogen-associated molecular patterns (PAMPs)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/23 06:01
PMCR- 2022/08/04
CRDT- 2022/08/22 04:31
PHST- 2022/06/08 00:00 [received]
PHST- 2022/06/30 00:00 [accepted]
PHST- 2022/08/22 04:31 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/23 06:01 [medline]
PHST- 2022/08/04 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.964112 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Aug 4;13:964112. doi: 10.3389/fmicb.2022.964112. 
      eCollection 2022.