PMID- 35993048
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220912
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2022
DP  - 2022
TI  - Integrated Analysis of Crucial Genes and miRNAs Associated with Osteoporotic 
      Fracture of Type 2 Diabetes.
PG  - 3921570
LID - 10.1155/2022/3921570 [doi]
LID - 3921570
AB  - PURPOSE: The aim of this study is to explore pathological mechanisms of bone 
      fragility in type 2 diabetes mellitus (T2DM) patients. METHODS: Identifying 
      common genes for T2DM and osteoporosis by taking the intersection is shared by 
      the Comparative Toxicogenomics Database (CTD), DISEASES, and GeneCards databases. 
      The differentially expressed genes (DEGs) and the differentially expressed miRNAs 
      (DEMs) were identified by analyzing the Gene Expression Omnibus (GEO) datasets 
      (GSE35958, GSE43950, and GSE70318). FunRich and miRNet were applied to predict 
      potential upstream transcription factors and downstream target genes of candidate 
      DEMs, respectively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and 
      Genomes (KEGG) enrichment analyses were performed to explore potential mechanisms 
      using Metascape. Eventually, a miRNA-gene network was constructed by Cytoscape 
      software. RESULTS: 271 common targets and 35 common DEGs between T2DM and 
      osteoporosis were screened out in the above databases, and a total of ten DEMs 
      were obtained in the GSE70318. SP1 was predicted to potentially regulate most of 
      the DEMs. Enrichment analysis showed the PI3K-Akt signaling pathway and AGE-RAGE 
      signaling pathway in diabetic complications may play an important role in 
      diabetic skeletal fragility. Two genes (NAMPT and IGFBP5) were considered as key 
      genes involving in the development of diabetic osteoporosis. Through the 
      construction of the miRNA-gene network, most of the hub genes were found to be 
      potentially modulated by miR-96-5p and miR-7-5p. CONCLUSION: The study uncovered 
      several important genes, miRNAs, and pathological mechanisms involved in diabetic 
      skeletal fragility, among which the PI3K-Akt signaling pathway and AGE-RAGE 
      signaling pathway in diabetic complications may play important roles.
CI  - Copyright (c) 2022 Liang Mo et al.
FAU - Mo, Liang
AU  - Mo L
AUID- ORCID: 0000-0002-5554-6196
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Wang, Zhangzheng
AU  - Wang Z
AUID- ORCID: 0000-0003-3871-5143
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Huang, Haoran
AU  - Huang H
AUID- ORCID: 0000-0002-6450-2486
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Li, Jianxiong
AU  - Li J
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Ma, Chao
AU  - Ma C
AD  - Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 51000 
      Guangdong Province, China.
FAU - Zhang, Jiahao
AU  - Zhang J
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Huang, Fayi
AU  - Huang F
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - He, Wei
AU  - He W
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Liu, Yuhao
AU  - Liu Y
AUID- ORCID: 0000-0002-0447-6677
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
FAU - Zhou, Chi
AU  - Zhou C
AUID- ORCID: 0000-0003-1905-9494
AD  - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405 Guangdong Province, China.
LA  - eng
PT  - Journal Article
DEP - 20220810
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (MIRN7 microRNA, human)
RN  - 0 (MIRN96 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Computational Biology
MH  - *Diabetes Mellitus, Type 2/complications/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Glycation End Products, Advanced/genetics/metabolism
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - *Osteoporosis/etiology/genetics/metabolism
MH  - *Osteoporotic Fractures/etiology/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Receptor for Advanced Glycation End Products/genetics/metabolism
PMC - PMC9385370
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/08/23 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/08/10
CRDT- 2022/08/22 04:37
PHST- 2022/01/05 00:00 [received]
PHST- 2022/07/17 00:00 [accepted]
PHST- 2022/08/22 04:37 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/08/10 00:00 [pmc-release]
AID - 10.1155/2022/3921570 [doi]
PST - epublish
SO  - Biomed Res Int. 2022 Aug 10;2022:3921570. doi: 10.1155/2022/3921570. eCollection 
      2022.