PMID- 35993285
OWN - NLM
STAT- MEDLINE
DCOM- 20221021
LR  - 20230823
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 34
IP  - 6
DP  - 2022 Nov
TI  - Detection of invasive Escherichia coli in dogs with granulomatous colitis using 
      immunohistochemistry.
PG  - 990-994
LID - 10.1177/10406387221119712 [doi]
AB  - Granulomatous colitis in dogs can be associated with infection of the colonic 
      mucosa by invasive strains of Escherichia coli. To date, fluorescence in situ 
      hybridization (FISH) is the gold-standard method to assess intramucosal and 
      intracellular bacterial invasion. However, FISH requires expensive fluorescence 
      microscopy equipment and is therefore not widely available. We investigated the 
      use of immunohistochemistry (IHC) as an alternative method to detect invasive E. 
      coli in dogs with granulomatous colitis. Archived paraffin-embedded blocks were 
      selected from 26 dogs with colitis, in which FISH had been performed by an 
      outside laboratory. Using a polyclonal antibody, IHC for E. coli was performed on 
      sections cut from the same blocks, and the presence of invasive E. coli was 
      recorded. All 11 specimens in which FISH had detected E. coli were also positive 
      on IHC, with strong immunolabeling in the cytoplasm of macrophages and 
      extracellularly in the lamina propria; all 15 specimens that were negative for 
      invasive bacteria on FISH were also negative on IHC. We found that IHC is a 
      sensitive technique for the detection of invasive E. coli in dogs with 
      granulomatous colitis.
FAU - Ishii, Patricia Eri
AU  - Ishii PE
AUID- ORCID: 0000-0002-9569-6404
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      College Station, TX, USA.
FAU - Suchodolski, Jan S
AU  - Suchodolski JS
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      College Station, TX, USA.
FAU - Duarte, Ricardo
AU  - Duarte R
AD  - Gastrovet, Sao Paulo, Brazil.
FAU - Pereira, Ana R C
AU  - Pereira ARC
AD  - School of Veterinary Medicine and Animal Science of University of Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Lidbury, Jonathan A
AU  - Lidbury JA
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      College Station, TX, USA.
FAU - Steiner, Joerg M
AU  - Steiner JM
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      College Station, TX, USA.
FAU - Giaretta, Paula R
AU  - Giaretta PR
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      College Station, TX, USA.
LA  - eng
PT  - Journal Article
DEP - 20220822
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Dogs
MH  - Animals
MH  - *Crohn Disease/microbiology/veterinary
MH  - Escherichia coli/genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - *Dog Diseases/diagnosis/microbiology
PMC - PMC9597337
OTO - NOTNLM
OT  - Escherichia coli
OT  - colitis
OT  - dogs
OT  - granulomatous colitis
OT  - histiocytic colitis
COIS- Patricia Eri Ishii, Paula R. Giaretta, Jonathan A. Lidbury, Jan S. Suchodolski, 
      and Joerg M. Steiner are employed by the Gastrointestinal Laboratory at Texas A&M 
      University, which offers laboratory tests, including gastrointestinal 
      histopathology services, on a fee-for-service basis.
EDAT- 2022/08/23 06:00
MHDA- 2022/10/22 06:00
PMCR- 2023/08/22
CRDT- 2022/08/22 05:33
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/10/22 06:00 [medline]
PHST- 2022/08/22 05:33 [entrez]
PHST- 2023/08/22 00:00 [pmc-release]
AID - 10.1177_10406387221119712 [pii]
AID - 10.1177/10406387221119712 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2022 Nov;34(6):990-994. doi: 10.1177/10406387221119712. Epub 
      2022 Aug 22.