PMID- 35993956
OWN - NLM
STAT- MEDLINE
DCOM- 20220824
LR  - 20220826
IS  - 2162-6537 (Electronic)
IS  - 0731-8898 (Linking)
VI  - 41
IP  - 3
DP  - 2022
TI  - miR-1246 Promotes Laryngeal Squamous Cell Carcinoma Progression by Interacting 
      with THBS1.
PG  - 65-75
LID - 10.1615/JEnvironPatholToxicolOncol.2022040516 [doi]
AB  - MicroRNAs (miRNAs) have been confirmed to be related to the occurrence and 
      progress of multiple cancers, including laryngeal squamous cell carcinoma (LSCC). 
      The present work focused on exploring the role of miR-1246 in LSCC and 
      investigating the possible mechanisms. miR-1246 expression levels within clinical 
      LSCC tissues and cell lines (TU212 and AMC-HN-8) were detected through 
      quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. 
      Then the overall survival (OS) rates of LSCC patients with different miR-1246 
      expressions were assessed by the Kaplan-Meier method. In addition, the roles of 
      miR-1246 in the proliferation, apoptosis and migration of TU212 and AMC-HN-8 
      cells were measured by colony formation, flow cytometry, wound-healing, and 
      Western blot (WB) assays, respectively. Moreover, TargetScan was carried out to 
      predict the miR-1246 targets (thrombospondin-1, THBS1), further confirmed by a 
      dual-luciferase reporter assay. Afterward, the negative relationship between 
      miR-1246 and THBS1 was assessed by qRT-PCR and WB. Furthermore, the restoring 
      effects of THBS1 on TU212 and AMC-HN-8 functional roles transfected with miR-1246 
      inhibitor were further investigated. miR-1246 expression levels were increased in 
      clinical LSCC tissues and cell lines. Patients with low miR-1246 expression 
      exhibited improved OS rates. In addition, miR-1246 down-regulation notably 
      suppressed cell proliferation and migration and induced cell apoptosis of TU212 
      and AMC-HN-8 cells. Moreover, THBS1 was predicted and confirmed as a direct 
      target of miR-1246 and negatively related to miR-1246 expression. Mechanically, 
      THBS1 inhibition partially rescued the effects of the miR-1246 inhibitor on 
      proliferation, apoptosis and migration of TU212 and AMC-HN-8 cells. This study 
      provides an experimental basis suggesting the potential of miR-1246/THBS1 as the 
      novel markers for LSCC.
FAU - Wu, Lifeng
AU  - Wu L
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
FAU - Zuo, Na
AU  - Zuo N
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
FAU - Pan, Shuo
AU  - Pan S
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
FAU - Wang, Qixue
AU  - Wang Q
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
FAU - Ma, Jun
AU  - Ma J
AD  - Department of Otolaryngology-Head and Neck Surgery, Yijisan Hospital of Wannan 
      Medical College, Wuhu 241001, Anhui, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Environ Pathol Toxicol Oncol
JT  - Journal of environmental pathology, toxicology and oncology : official organ of 
      the International Society for Environmental Toxicology and Cancer
JID - 8501420
RN  - 0 (MIRN1246 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Thrombospondin 1)
RN  - 0 (thrombospondin-1, human)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Thrombospondin 1/*metabolism
EDAT- 2022/08/23 06:00
MHDA- 2022/08/25 06:00
CRDT- 2022/08/22 10:32
PHST- 2022/08/22 10:32 [entrez]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/08/25 06:00 [medline]
AID - 1ceeed0b6c67b42f,3460534b76e1fbad [pii]
AID - 10.1615/JEnvironPatholToxicolOncol.2022040516 [doi]
PST - ppublish
SO  - J Environ Pathol Toxicol Oncol. 2022;41(3):65-75. doi: 
      10.1615/JEnvironPatholToxicolOncol.2022040516.