PMID- 35994839
OWN - NLM
STAT- MEDLINE
DCOM- 20221101
LR  - 20221101
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 152
DP  - 2022 Dec 20
TI  - Evaluation of the ACTH stimulation test using a low dose of a depot formulation 
      in healthy dogs and in dogs with untreated Cushing's syndrome.
PG  - 207-211
LID - S0034-5288(22)00243-0 [pii]
LID - 10.1016/j.rvsc.2022.08.010 [doi]
AB  - The sensitivity of the adrenocorticotropic hormone (ACTH) stimulation test to 
      detect Cushing's Syndrome (CS) using a depot formulation needs to be evaluated. 
      The aims of this study were to propose a reference interval (RI) for cortisol 
      values 1-hour after administration of a low-dose of depot ACTH in healthy dogs, 
      and to evaluate the sensitivity of this test to detect CS, differentiating among 
      types of CS based on ultrasound findings. Forty-one healthy dogs (20 males, 21 
      females) were prospectively included. Additionally, 90 dogs with CS (31 males, 59 
      females) were retrospectively included. Dogs with CS were ultrasonographically 
      classified as follows: 44 dogs with symmetrical adrenomegaly consistent with 
      pituitary-dependent hypercortisolism (PDH), 8 dogs with unilateral adrenomegaly 
      and atrophy of the contralateral adrenal gland or unilateral or bilateral 
      adrenomegaly with malignancy features consistent with adrenal-dependent 
      hypercortisolism (ADH), 34 dogs with equivocal adrenal asymmetry (EAA) and 4 dogs 
      with normal adrenal thickness. In healthy dogs, lower and upper limit of the 95% 
      RI for 1-hour post-ACTH cortisol concentration and their 90% confidence 
      intervals, were 4.4 (2.7-5.8) mug/dl and 18.4 (16.5-20.0) mug/dl, respectively. 
      Post-ACTH cortisol concentration was above the RI in 90.0% (ci95%, 76.1-100) of 
      dogs with CS. An elevated post-ACTH cortisol concentration was detected in 95.5% 
      (ci95%, 76.1-100) of dogs with PDH, 62.5% (ci95%, 46.1-78.9) of dogs with ADH and 
      88.2% (ci95%, 69.1-100) of dogs with EAA. The sensitivity of the ACTH stimulation 
      test using a low-dose of depot ACTH in high in dogs with CS.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Melian, Carlos
AU  - Melian C
AD  - Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las 
      Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain; University Institute of 
      Biomedical and Health Research, Paseo Blas Cabrera Felipe s/n, 35016 Las Palmas 
      de Gran Canaria, Spain.
FAU - Blanco, Beatriz
AU  - Blanco B
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      University of Cordoba, 14005 Cordoba, Spain.
FAU - Ginel, Pedro J
AU  - Ginel PJ
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      University of Cordoba, 14005 Cordoba, Spain.
FAU - Perez-Lopez, Laura
AU  - Perez-Lopez L
AD  - University Institute of Biomedical and Health Research, Paseo Blas Cabrera Felipe 
      s/n, 35016 Las Palmas de Gran Canaria, Spain. Electronic address: 
      laura.perezlopez@ulpgc.es.
LA  - eng
PT  - Journal Article
DEP - 20220812
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Male
MH  - Female
MH  - Dogs
MH  - Animals
MH  - *Cushing Syndrome/diagnosis/veterinary
MH  - Adrenocorticotropic Hormone/pharmacology
MH  - Hydrocortisone
MH  - Retrospective Studies
MH  - *Pituitary ACTH Hypersecretion/veterinary
MH  - *Dog Diseases/diagnostic imaging
OTO - NOTNLM
OT  - Adrenal gland
OT  - Adrenocorticotropic hormone
OT  - Cushing's syndrome
OT  - Hypercortisolism
COIS- Declaration of Competing Interest None.
EDAT- 2022/08/23 06:00
MHDA- 2022/11/02 06:00
CRDT- 2022/08/22 18:15
PHST- 2021/09/28 00:00 [received]
PHST- 2022/05/19 00:00 [revised]
PHST- 2022/08/08 00:00 [accepted]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/08/22 18:15 [entrez]
AID - S0034-5288(22)00243-0 [pii]
AID - 10.1016/j.rvsc.2022.08.010 [doi]
PST - ppublish
SO  - Res Vet Sci. 2022 Dec 20;152:207-211. doi: 10.1016/j.rvsc.2022.08.010. Epub 2022 
      Aug 12.