PMID- 35995180
OWN - NLM
STAT- MEDLINE
DCOM- 20221014
LR  - 20221014
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 220
DP  - 2022 Nov 1
TI  - In-depth investigation of the hypoglycemic mechanism of Morchella importuna 
      polysaccharide via metabonomics combined with 16S rRNA sequencing.
PG  - 659-670
LID - S0141-8130(22)01820-7 [pii]
LID - 10.1016/j.ijbiomac.2022.08.117 [doi]
AB  - Increasing evidence indicates that type 2 diabetes mellitus (T2DM) is closely 
      related to intestinal bacteria disorders and abnormal hepatic metabolism. 
      Morchella importuna polysaccharide (MIP) shows excellent hypoglycemic activity in 
      vitro. However, the hypoglycemic effect and mechanism of MIP in vivo have yet to 
      be investigated. In this study, the blood glucose, blood lipid and insulin 
      resistance of diabetic mice after MIP intervention were measured to evaluate its 
      hypoglycemic effect. Then, the microbiome and metabolomics were combined to 
      explore the hypoglycemic mechanism of MIP. Results indicated that high dose MIP 
      (400 mg/kg) had significant hypoglycemic effect. Furthermore, MIP could reverse 
      diabetes-induced intestinal disorder by increasing the abundance of Akkermansia, 
      Blautia, Dubosiella, and Lachnospiraceae, as well as decreasing the abundance of 
      Helicobacteraceae. Besides, the hepatic metabolites and complex network systems 
      formed by multiple metabolic pathways were regulated after MIP treatment. 
      Notably, a new biomarker of diabetes (N-P-coumaroyl spermidine) was discovered in 
      this study. Moreover, the significant association between intestinal bacteria and 
      hepatic metabolites was determined by correlations analysis, which in turn 
      confirmed MIP alleviated T2DM via the gut-liver axis. Therefore, these findings 
      elucidated in-depth hypoglycemic mechanisms of MIP and provided a new biomarker 
      for the prevention of diabetes.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Pan, Xu
AU  - Pan X
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
FAU - Meng, Junlong
AU  - Meng J
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Engineering Research Center of Edible Fungi, Taigu, 
      Shanxi 030801, China. Electronic address: mengjunlongseth@hotmail.com.
FAU - Xu, Lijing
AU  - Xu L
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, 
      Taigu, Shanxi 030801, China. Electronic address: xulijingsx@hotmail.com.
FAU - Chang, Mingchang
AU  - Chang M
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Engineering Research Center of Edible Fungi, Taigu, 
      Shanxi 030801, China.
FAU - Feng, Cuiping
AU  - Feng C
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, 
      Taigu, Shanxi 030801, China.
FAU - Geng, Xueran
AU  - Geng X
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, 
      Taigu, Shanxi 030801, China.
FAU - Cheng, Yanfen
AU  - Cheng Y
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, 
      Taigu, Shanxi 030801, China.
FAU - Guo, Dongdong
AU  - Guo D
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
FAU - Liu, Rongzhu
AU  - Liu R
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
FAU - Wang, Zhichao
AU  - Wang Z
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
FAU - Li, Dongjie
AU  - Li D
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
FAU - Tan, Lirui
AU  - Tan L
AD  - College of Food Science and Engineering, Shanxi Agricultural University, Taigu, 
      Shanxi 030801, China.
LA  - eng
PT  - Journal Article
DEP - 20220819
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (N(4)-(2-propenal)spermidine)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - U87FK77H25 (Spermidine)
RN  - Morchella importuna
SB  - IM
MH  - Animals
MH  - Ascomycota
MH  - Biomarkers
MH  - Blood Glucose/metabolism
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - *Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Dietary Carbohydrates/therapeutic use
MH  - Hypoglycemic Agents/therapeutic use
MH  - Metabolomics
MH  - Mice
MH  - Polysaccharides/therapeutic use
MH  - RNA, Ribosomal, 16S/genetics
MH  - Spermidine/analogs & derivatives
OTO - NOTNLM
OT  - Hypoglycemic
OT  - Intestinal bacteria
OT  - Metabolomics
OT  - Morchella importuna polysaccharides
OT  - Type 2 diabetes mellitus
COIS- Declaration of competing interest There are no conflicts of interest to declare.
EDAT- 2022/08/23 06:00
MHDA- 2022/10/15 06:00
CRDT- 2022/08/22 19:33
PHST- 2022/06/28 00:00 [received]
PHST- 2022/08/11 00:00 [revised]
PHST- 2022/08/16 00:00 [accepted]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/10/15 06:00 [medline]
PHST- 2022/08/22 19:33 [entrez]
AID - S0141-8130(22)01820-7 [pii]
AID - 10.1016/j.ijbiomac.2022.08.117 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 Nov 1;220:659-670. doi: 10.1016/j.ijbiomac.2022.08.117. 
      Epub 2022 Aug 19.