PMID- 35995508
OWN - NLM
STAT- MEDLINE
DCOM- 20220923
LR  - 20230916
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 209
IP  - 6
DP  - 2022 Sep 15
TI  - Migrating Type 2 Dendritic Cells Prime Mucosal Th17 Cells Specific to Small 
      Intestinal Commensal Bacteria.
PG  - 1200-1211
LID - 10.4049/jimmunol.2200204 [doi]
LID - ji2200204
AB  - Dendritic cells (DCs) are professional APCs equipped with MHC-restricted Ags, 
      costimulations, and cytokines that effectively prime and differentiate naive T 
      cells into distinct functional subsets. The immune signals that DCs carry reflect 
      the route of Ag uptake and the innate stimuli they received. In the mucosal 
      tissues, owing to the great variety of foreign Ags and inflammatory cues, DCs are 
      predominantly activated and migratory. In the small intestine, CD4 Th17 cells are 
      abundant and have been shown to be regulated by DCs and macrophages. Using a 
      mouse commensal bacteria experimental model, we identified that the early priming 
      step of commensal-driven Th17 cells is controlled by bona fide Zbtb46-expressing 
      DCs. CCR7-dependent migration of type 2 DCs (DC2s) from the small intestine to 
      the mesenteric lymph nodes (MLNs) is essential for the activation of naive CD4 T 
      cells. The migratory DC2 population in the MLNs is almost exclusively Esam(+) 
      cells. Single-cell RNA sequencing highlighted the abundance of costimulatory 
      markers (CD40 and OX40) and chemokines (Ccl22 and Cxcl16) on MLN migratory DCs. 
      Further resolution of MLN migratory DC2s revealed that the Th17-polarizing 
      cytokine IL-6 colocalizes with DC2s expressing CD40, Ccl17, and Ccl22. Thus, 
      early Th17 cell differentiation is initiated by a small subset of migratory DC2s 
      in the gut-draining lymph nodes.
CI  - Copyright (c) 2022 by The American Association of Immunologists, Inc.
FAU - Ngoi, Soo
AU  - Ngoi S
AD  - Division of Hematology, Department of Internal Medicine, The Ohio State 
      University, Columbus, OH; and bei.liu@osumc.edu soomun.ngoi@osumc.edu.
FAU - Yang, Yi
AU  - Yang Y
AD  - Department of Microbiology and Immunology, Hollings Cancer Center, Medical 
      University of South Carolina, Charleston, SC.
FAU - Iwanowycz, Stephen
AU  - Iwanowycz S
AD  - Department of Microbiology and Immunology, Hollings Cancer Center, Medical 
      University of South Carolina, Charleston, SC.
FAU - Gutierrez, Jennifer
AU  - Gutierrez J
AD  - Department of Microbiology and Immunology, Hollings Cancer Center, Medical 
      University of South Carolina, Charleston, SC.
FAU - Li, Yingqi
AU  - Li Y
AD  - Department of Microbiology and Immunology, Hollings Cancer Center, Medical 
      University of South Carolina, Charleston, SC.
FAU - Williams, Christina
AU  - Williams C
AD  - Department of Microbiology and Immunology, Hollings Cancer Center, Medical 
      University of South Carolina, Charleston, SC.
FAU - Hill, Megan
AU  - Hill M
AD  - Division of Hematology, Department of Internal Medicine, The Ohio State 
      University, Columbus, OH; and.
FAU - Chung, Dongjun
AU  - Chung D
AD  - Division of Hematology, Department of Internal Medicine, The Ohio State 
      University, Columbus, OH; and.
FAU - Allen, Carter
AU  - Allen C
AD  - Division of Hematology, Department of Internal Medicine, The Ohio State 
      University, Columbus, OH; and.
FAU - Liu, Bei
AU  - Liu B
AD  - Division of Hematology, Department of Internal Medicine, The Ohio State 
      University, Columbus, OH; and bei.liu@osumc.edu soomun.ngoi@osumc.edu.
LA  - eng
GR  - R01 CA193939/CA/NCI NIH HHS/United States
GR  - U01 AI125859/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220822
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Bacteria
MH  - Chemokines
MH  - Cytokines
MH  - *Dendritic Cells
MH  - Interleukin-6
MH  - Intestine, Small
MH  - Lymph Nodes
MH  - Mucous Membrane
MH  - Receptors, CCR7
MH  - *Th17 Cells
PMC - PMC9492644
MID - NIHMS1824349
COIS- COMPETING INTEREST STATEMENT The authors declare no competing interests
EDAT- 2022/08/23 06:00
MHDA- 2022/09/24 06:00
PMCR- 2023/09/15
CRDT- 2022/08/22 21:04
PHST- 2022/03/18 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/08/23 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
PHST- 2022/08/22 21:04 [entrez]
PHST- 2023/09/15 00:00 [pmc-release]
AID - jimmunol.2200204 [pii]
AID - 10.4049/jimmunol.2200204 [doi]
PST - ppublish
SO  - J Immunol. 2022 Sep 15;209(6):1200-1211. doi: 10.4049/jimmunol.2200204. Epub 2022 
      Aug 22.