PMID- 35997119
OWN - NLM
STAT- MEDLINE
DCOM- 20220824
LR  - 20220919
IS  - 1045-4403 (Print)
IS  - 1045-4403 (Linking)
VI  - 32
IP  - 6
DP  - 2022
TI  - LncRNA ZFPM2-AS1 Enhances Retinoblastoma Development by Targeting the 
      miR-3612/NT5E Signaling Axis.
PG  - 69-82
LID - 10.1615/CritRevEukaryotGeneExpr.2022042697 [doi]
AB  - Background - Retinoblastoma (RB) is an intraocular malignancy usually observed in 
      children. The zinc finger protein, FOG family member 2 (ZFPM2)-antisense RNA 1 
      (AS1), an anomalous long non-coding RNA, is detected in many cancers. This study 
      aimed to evaluate the role and underlying mechanism of ZFPM2-AS1 in RB. Methods - 
      ZFPM2-AS1, 5'-nucleotidase ecto (NT5E), and microRNA (miR)-3612 expression levels 
      were determined in RB cells and tissues. Multiple experiments, including cell 
      counting kit-8, 5'-bromo-2'-deoxyuridine, transwell, and enzyme-linked 
      immunosorbent assays, were conducted to evaluate their effects on cell 
      proliferation, migration, and apoptosis. Additionally, the 
      ZFPM2-AS1/miR-3612/NT5E axis was validated using luciferase reporter, anti-AGO2 
      RNA immunoprecipitation, and RNA pull-down assays. Results - Overexpression of 
      ZFPM2-AS1 and NT5E and downregulation of miR-3612 expression levels were observed 
      in RB cells and tissues. Knockdown of ZFPM2-AS1 decreased the proliferation and 
      migration of RB cells, while enhancing their apoptosis. The 
      ZFPM2-AS1/miR-3612/NT5E axis was further supported by the negative correlation 
      between miR-3612 expression levels and ZFPM2-AS1 or NT5E expression levels in RB 
      tissues. Moreover, the miR-3612 inhibitor restored the inhibitory effect on the 
      malignant behavior of RB cells by silencing ZFPM2-AS1. Furthermore, NT5E 
      silencing phenocopied the effect of ZFPM2-AS1 silencing and abolished miR-3612 
      inhibitor-induced stimulation of the malignant behavior of RB cells. Conclusion - 
      These results suggest that by targeting the miR-3612/NT5E axis, ZFPM2-AS1 
      stimulates the proliferation and migration of RB cells, while inhibiting their 
      apoptosis. Therefore, ZFPM2-AS1 may be a promising therapeutic target associated 
      with RB development.
FAU - Ai, Kui
AU  - Ai K
AD  - Department of Pediatrics, Wuhan Third Hospital Guanggu district, Wuhan 430074, 
      Hubei, China.
FAU - Ni, Wenchang
AU  - Ni W
AD  - Department of Pediatrics, Wuhan Third Hospital Guanggu district, Wuhan 430074, 
      Hubei, China.
FAU - Li, Zhen
AU  - Li Z
AD  - Department of Pediatrics, Wuhan Third Hospital Guanggu district, Wuhan 430074, 
      Hubei, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Crit Rev Eukaryot Gene Expr
JT  - Critical reviews in eukaryotic gene expression
JID - 9007261
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (MIRN3612 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Antisense)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transcription Factors)
RN  - 0 (ZFPM2 protein, human)
RN  - EC 3.1.3.5 (5'-Nucleotidase)
RN  - EC 3.1.3.5 (NT5E protein, human)
SB  - IM
MH  - *5'-Nucleotidase/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Child
MH  - DNA-Binding Proteins/genetics
MH  - Family
MH  - *GPI-Linked Proteins/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - RNA, Antisense/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - *Retinoblastoma/genetics
MH  - Transcription Factors/genetics
MH  - Zinc Fingers
EDAT- 2022/08/24 06:00
MHDA- 2022/08/25 06:00
CRDT- 2022/08/23 06:03
PHST- 2022/08/23 06:03 [entrez]
PHST- 2022/08/24 06:00 [pubmed]
PHST- 2022/08/25 06:00 [medline]
AID - 40058b3a3fcd229f,2e5d55e5799acb4f [pii]
AID - 10.1615/CritRevEukaryotGeneExpr.2022042697 [doi]
PST - ppublish
SO  - Crit Rev Eukaryot Gene Expr. 2022;32(6):69-82. doi: 
      10.1615/CritRevEukaryotGeneExpr.2022042697.