PMID- 35997581
OWN - NLM
STAT- MEDLINE
DCOM- 20221114
LR  - 20231213
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 37
IP  - 12
DP  - 2022 Dec
TI  - hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage 
      M2 polarization via regulating MIF-PI3K-AKT signaling pathway.
PG  - 2819-2831
LID - 10.1002/tox.23639 [doi]
AB  - In the previous study, we have proved that exosomal miR-451 from human umbilical 
      cord mesenchymal stem cells (hUC-MSCs) attenuated burn-induced acute lung injury 
      (ALI). However, the mechanism of exosomal miR-451 in ALI remains unclear. 
      Therefore, this study aimed to study the molecular mechanism of hUC-MSCs-derived 
      exosomal miR-451 on ALI by regulating macrophage polarization. Exosomes were 
      isolated and identified by transmission electron microscope (TEM) and 
      nanoparticle tracking analysis (NTA). The expression of miR-451, macrophage 
      migration inhibitory factor (MIF) and PI3K/AKT signaling pathway proteins were 
      detected by qRT-PCR and western blot. Flow cytometry was used to detect the CD80 
      and CD206 positive cells. Severe burn rat model was established and HE was used 
      to detect the inflammatory cell infiltration and inflammatory injury. Dual 
      luciferase reporter system was used to detect the regulation of miR-451 to MIF. 
      The contents of cytokines were detected by ELISA. The results showed that 
      hUC-MSCs exosomes promoted macrophage M1 to M2 polarization. Furthermore, 
      hUC-MSCs-derived exosomal miR-451 alleviated ALI development and promoted 
      macrophage M1 to M2 polarization. Moreover, MIF was a direct target of miR-451. 
      Downregulation of MIF regulated by miR-451 alleviated ALI development promoted 
      macrophage M1 to M2 polarization. In addition, we found that MIF and 
      hUC-MSCs-derived exosomal miR-451 participated in ALI by regulating PI3K/AKT 
      signaling pathway. In conclusion, we indicated that hUC-MSCs-derived exosomal 
      miR-451 alleviated ALI by modulating macrophage M2 polarization via regulating 
      MIF-PI3K-AKT signaling pathway, which provided great scientific significance and 
      clinical application value for the treatment of burn-induced ALI.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Liu, Jisong
AU  - Liu J
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - Xing, Fuxi
AU  - Xing F
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - Fu, Quanyou
AU  - Fu Q
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - He, Bo
AU  - He B
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - Jia, Zhigang
AU  - Jia Z
AD  - Department of Burn and Plastic Surgery, Affiliated Hospital of Jiangnan 
      University, Wuxi, Jiangsu, China.
FAU - Du, Juan
AU  - Du J
AD  - Department of Minimally Invasive Surgery, Third Hospital of Bengbu, Bengbu, 
      Anhui, China.
FAU - Li, Yong
AU  - Li Y
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - Zhang, Xiangzhou
AU  - Zhang X
AD  - Department of Burn and Plastic Surgery, Third Hospital of Bengbu, Bengbu, Anhui, 
      China.
FAU - Chen, Xulin
AU  - Chen X
AUID- ORCID: 0000-0002-5182-2579
AD  - Department of Burns, First Affiliated Hospital of Anhui Medical University, 
      Hefei, Anhui, China.
LA  - eng
GR  - ahwj2021a042/Key scientific research project of Anhui Provincial Health 
      Commission in 2021/
GR  - The tenth batch of "3221" industrial innovation and scientific research projects 
      in Bengbu City/
PT  - Journal Article
DEP - 20220823
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - 0 (MicroRNAs)
RN  - EC 5.3.2.1 (MIF protein, human)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - 0 (MIRN451 microRNA, human)
RN  - EC 5.3.2.1 (Mif protein, rat)
RN  - 0 (MIRN451A microRNA, rat)
SB  - IM
MH  - Rats
MH  - Humans
MH  - Animals
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *Acute Lung Injury/genetics/metabolism
MH  - Signal Transduction/genetics
MH  - Macrophages/metabolism
MH  - *Burns/genetics
MH  - *Macrophage Migration-Inhibitory Factors/genetics/metabolism
MH  - Intramolecular Oxidoreductases/genetics/metabolism
OTO - NOTNLM
OT  - MIF
OT  - PI3K/AKT signaling pathway
OT  - acute lung injury
OT  - exosomal miR-451
OT  - hUC-MSCs
EDAT- 2022/08/24 06:00
MHDA- 2022/11/15 06:00
CRDT- 2022/08/23 09:43
PHST- 2022/08/01 00:00 [revised]
PHST- 2022/03/23 00:00 [received]
PHST- 2022/08/04 00:00 [accepted]
PHST- 2022/08/24 06:00 [pubmed]
PHST- 2022/11/15 06:00 [medline]
PHST- 2022/08/23 09:43 [entrez]
AID - 10.1002/tox.23639 [doi]
PST - ppublish
SO  - Environ Toxicol. 2022 Dec;37(12):2819-2831. doi: 10.1002/tox.23639. Epub 2022 Aug 
      23.