PMID- 36000350
OWN - NLM
STAT- MEDLINE
DCOM- 20220825
LR  - 20220826
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 41
DP  - 2022 Jan-Dec
TI  - The protective effects of ritodrine against hypoxia/reoxygenation-induced injury 
      in endometrial stromal cells.
PG  - 9603271221120650
LID - 10.1177/09603271221120650 [doi]
AB  - Endometriosis (EMS) is often observed in women of childbearing age and 
      significantly impacts patients' quality of life. Ritodrine is a beta2 receptor 
      agonist applied for relaxing the uterine smooth muscle. Its inhibitory effects on 
      inflammation have recently been noted. The present study explored the protective 
      impact of Ritodrine on hypoxia/reoxygenation (H/R)- induced injury in endometrial 
      stromal cells (ESCs). Human ESCs (HESCs) were treated with Ritodrine (0.1, 
      0.5 muM) for 24 h, followed by exposure to H/R for 6 h. Ritodrine ameliorated 
      H/R-induced higher reactive oxygen species (ROS), declined glutathione (GSH) 
      concentration and increased production of tumor necrosis factor-alpha (TNF-alpha), 
      interleukin- 6 (IL-6), and monocyte chemotactic protein 1 (MCP-1) in HESCs. 
      Furthermore, Ritodrine ameliorated the H/R-induced higher nuclear level of 
      nuclear factor kappa-B (NF-kappaB) p65 expression and increased luciferase activity of 
      the NF-kappaB promoter. In addition, we show that Ritodrine mitigated H/R-induced 
      higher estrogen receptor alpha (ER-alpha) expression in HESCs. Interestingly, 
      overexpressing ER-alpha abolished the regulatory effects of Ritodrine on oxidative 
      stress and the NF-kappaB pathway-mediated inflammation. Collectively, our data reveal 
      that Ritodrine alleviated H/R-induced injury in ESCs by inhibiting the ER-alpha/NF-kappaB 
      pathway.
FAU - Zhu, Jing
AU  - Zhu J
AD  - Department of Obstetrics and Gynecology, Nantong Maternity and Child Health Care 
      Hospital, China.
FAU - Liu, Haiyun
AU  - Liu H
AD  - Department of Obstetrics and Gynecology, Nantong Maternity and Child Health Care 
      Hospital, China.
FAU - Mao, Lijing
AU  - Mao L
AUID- ORCID: 0000-0002-5980-9459
AD  - Department of Obstetrics and Gynecology, Nantong Maternity and Child Health Care 
      Hospital, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - I0Q6O6740J (Ritodrine)
SB  - IM
MH  - Female
MH  - Humans
MH  - Hypoxia/drug therapy
MH  - Inflammation/metabolism
MH  - Interleukin-6/metabolism
MH  - *NF-kappa B/metabolism
MH  - Oxidation-Reduction
MH  - Quality of Life
MH  - *Ritodrine/metabolism/pharmacology
MH  - Stromal Cells
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Endometriosis
OT  - Ritodrine
OT  - estrogen receptor alpha
OT  - nuclear factor kappa-B
OT  - oxidative stress
EDAT- 2022/08/25 06:00
MHDA- 2022/08/26 06:00
CRDT- 2022/08/24 04:33
PHST- 2022/08/24 04:33 [entrez]
PHST- 2022/08/25 06:00 [pubmed]
PHST- 2022/08/26 06:00 [medline]
AID - 10.1177/09603271221120650 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221120650. doi: 
      10.1177/09603271221120650.