PMID- 36000381
OWN - NLM
STAT- MEDLINE
DCOM- 20221121
LR  - 20221121
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 133
IP  - 6
DP  - 2022 Dec
TI  - Antimicrobial effects of automobile screen washes against Legionella pneumophila.
PG  - 3596-3604
LID - 10.1111/jam.15793 [doi]
AB  - AIMS: Legionella pneumophila (Lp), a human pathogen, has been detected in 
      windscreen wiper fluid reservoirs (WWFRs) where commercial screen washes (CSWs) 
      are commonly added. Limited information is available on CSWs against planktonic 
      Lp; however, responses of sessile Lp and planktonic Lp pre-acclimated in 
      nutrient-limited water to CSWs remain unknown. This study thus investigates the 
      antibacterial effects of CSWs on sessile and starved planktonic Lp, in comparison 
      with unstarved Lp. METHODS AND RESULTS: Lp biofilms were produced on glass and 
      WWFR materials of high-density polyethylene (HDPE) and polypropylene (PP). 
      Planktonic Lp with and without acclimation in tap water were prepared. Log 
      reductions in cell counts averaged 0.4-5.0 for 10 brands of CSWs against sessile 
      Lp and 1.0-3.9 and 0.9-4.9, respectively, against starved and unstarved 
      planktonic Lp for five CSWs. Both biofilm formation and acclimation in tap water 
      enhanced Lp resistance to CSWs. Significantly different log-reduction values 
      among CSW brands were observed for sessile Lp on HDPE and planktonic Lp 
      regardless of acclimation (p < 0.05). CONCLUSIONS: Biofilm formation, starvation 
      acclimation and CSW brand are crucial factors influencing Lp response to CSWs. 
      SIGNIFICANCE AND IMPACT OF STUDY: This study advances the knowledge of Lp 
      reaction in anthropogenic water systems with CSWs.
CI  - (c) 2022 Society for Applied Microbiology.
FAU - Hsiao, Yun-Chung
AU  - Hsiao YC
AD  - Department of Public Health, National Taiwan University, Taipei, Taiwan.
FAU - Hung, Yu-Hsin
AU  - Hung YH
AD  - Department of Public Health, National Taiwan University, Taipei, Taiwan.
FAU - Horng, Yu-Ju
AU  - Horng YJ
AD  - Institute of Environmental and Occupational Health Sciences, National Taiwan 
      University, Taipei, Taiwan.
FAU - Chang, Ching-Wen
AU  - Chang CW
AUID- ORCID: 0000-0003-4446-837X
AD  - Department of Public Health, National Taiwan University, Taipei, Taiwan.
AD  - Institute of Environmental and Occupational Health Sciences, National Taiwan 
      University, Taipei, Taiwan.
LA  - eng
GR  - 106-2314-B-002-087-MY3/Ministry of Science and Technology, Taiwan/
PT  - Journal Article
DEP - 20220907
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 9002-88-4 (Polyethylene)
RN  - 059QF0KO0R (Water)
RN  - 0 (Anti-Infective Agents)
SB  - IM
MH  - Humans
MH  - *Legionella pneumophila
MH  - Automobiles
MH  - Polyethylene
MH  - Biofilms
MH  - Plankton
MH  - Water/pharmacology
MH  - *Anti-Infective Agents/pharmacology
MH  - Water Microbiology
OTO - NOTNLM
OT  - Legionella pneumophila
OT  - planktonic
OT  - screen washes
OT  - sessile
OT  - starvation
EDAT- 2022/08/25 06:00
MHDA- 2022/11/22 06:00
CRDT- 2022/08/24 04:53
PHST- 2022/08/17 00:00 [revised]
PHST- 2022/05/17 00:00 [received]
PHST- 2022/08/20 00:00 [accepted]
PHST- 2022/08/25 06:00 [pubmed]
PHST- 2022/11/22 06:00 [medline]
PHST- 2022/08/24 04:53 [entrez]
AID - 10.1111/jam.15793 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2022 Dec;133(6):3596-3604. doi: 10.1111/jam.15793. Epub 2022 
      Sep 7.