PMID- 36004694
OWN - NLM
STAT- MEDLINE
DCOM- 20220826
LR  - 20220919
IS  - 1045-4403 (Print)
IS  - 1045-4403 (Linking)
VI  - 32
IP  - 7
DP  - 2022
TI  - Kruppel Like Factor 5 Enhances High Glucose-Induced Renal Tubular Epithelial Cell 
      Transdifferentiation in Diabetic Nephropathy.
PG  - 35-45
LID - 10.1615/CritRevEukaryotGeneExpr.2022043565 [doi]
AB  - Background - Diabetic nephropathy (DN) is a principal reason for kidney disease 
      worldwide. High glucose (HG) is a major factor for DN. Kruppel like factor 5 
      (KLF5) participates in DN development. In the present study, we aim to elaborate 
      the role of KLF5 in HG-induced renal tubular epithelial cell (RTEC) 
      transdifferentiation in DN. Methods - RTECs (HK-2 cells) were treated with HG and 
      were transfected with si-KLF5 or oe-HMGB1. Afterwards, expression of KLF5 and 
      HMGB1 was detected, HK cell viability was determined, and levels of alpha-smooth 
      muscle actin (alpha-SMA), E-cadherin, vimentin, and transforming growth factor beta 1 
      (TGF-beta1) were assessed. Additionally, the binding relation between KLF5 and HMGB1 
      was analyzed. Results - In HK-2 cells with HG treatment, expression of KLF5 and 
      HMGB1 was upregulated; levels of alpha-SMA, vimentin, and TGF-beta1 were increased; and 
      E-cadherin level was decreased. Moreover, KLF5 silencing resulted in 
      down-regulated levels of alpha-SMA, vimentin, and TGF-beta1 but upregulated level of 
      E-cadherin. On the other hand, KLF5 could bind to the HMGB1 promoter and activate 
      HMGB1 transcription. HMGB1 overexpression partially counteracted the inhibitive 
      effect of KLF5 silencing on HG-induced HK-2 transdifferentiation. Conclusion - HG 
      induced overexpressed KLF5 in RTECs, and as a transcription factor, KLF5 could 
      bind to the HMGB1 promoter, thereby promoting HMGB1 transcription and RTEC 
      transdifferentiation.
FAU - Zou, Hongchang
AU  - Zou H
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Zhu, Shuying
AU  - Zhu S
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Chen, Yanxia
AU  - Chen Y
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Cai, Ni
AU  - Cai N
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Xu, Chengyun
AU  - Xu C
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Tu, Weiping
AU  - Tu W
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
FAU - Qin, Xiaohua
AU  - Qin X
AD  - Department of Nephrology, The Second Affiliated Hospital of Nanchang University, 
      Nanchang 330006, Jiangxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Crit Rev Eukaryot Gene Expr
JT  - Critical reviews in eukaryotic gene expression
JID - 9007261
RN  - 0 (Cadherins)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Vimentin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cadherins/genetics/metabolism
MH  - Cell Transdifferentiation/genetics
MH  - *Diabetes Mellitus
MH  - *Diabetic Nephropathies/genetics/metabolism
MH  - Epithelial Cells/metabolism
MH  - Glucose/metabolism/pharmacology
MH  - *HMGB1 Protein/metabolism/pharmacology
MH  - Humans
MH  - Kruppel-Like Transcription Factors/genetics/metabolism
MH  - Transcription Factors/metabolism
MH  - Transforming Growth Factor beta1/genetics/metabolism/pharmacology
MH  - Vimentin/genetics/metabolism/pharmacology
EDAT- 2022/08/26 06:00
MHDA- 2022/08/27 06:00
CRDT- 2022/08/25 05:43
PHST- 2022/08/25 05:43 [entrez]
PHST- 2022/08/26 06:00 [pubmed]
PHST- 2022/08/27 06:00 [medline]
AID - 0a7b625a5f38a24a,4d002c264b8639dd [pii]
AID - 10.1615/CritRevEukaryotGeneExpr.2022043565 [doi]
PST - ppublish
SO  - Crit Rev Eukaryot Gene Expr. 2022;32(7):35-45. doi: 
      10.1615/CritRevEukaryotGeneExpr.2022043565.