PMID- 36004974
OWN - NLM
STAT- MEDLINE
DCOM- 20220826
LR  - 20220828
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 12
IP  - 8
DP  - 2022 Jul 29
TI  - The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an 
      Antibody Chip Biosensor.
LID - 10.3390/bios12080578 [doi]
LID - 578
AB  - In the modern farming industry, the irrational or illegal use of veterinary drugs 
      leads to residues in animal-derived food, which can seriously threaten human 
      health. Efficient detection of low concentrations of drug residues in animal 
      products in a short time is a key challenge for analytical methods. This study 
      proposes to use an antibody chip biosensor for rapid and automated analysis of 
      cephalosporins, aminoglycosides, and sulfonamide antibiotics in pork and milk. 3D 
      polymer slides were applied for the preparation of antibody chips. Ovalbumin 
      (OVA) or bovine serum albumin (BSA) conjugates of the haptens were immobilized as 
      spots on disposable chips. Monoclonal antibodies (mAbs) against cefalexin, 
      ceftiofur, gentamicin, neomycin, and sulfonamides allowed the simultaneous 
      detection of the respective analytes. Antibody binding was detected by a second 
      antibody labeled with Cy3-generating fluorescence, which was scanned a with chip 
      scanner. The limits of detection (LOD) for all the analytes were far below the 
      respective maximum residue limits (MRLs) and ranged from 0.51 to 4.3 microg/kg. The 
      average recoveries of all the analytes in each sample were in the range of 
      81.6-113.6%. The intra- and inter-assay CV was less than 12.9% and showed good 
      accuracy and precision for all the antibiotics at the MRL level. The sample 
      pretreatment method is simple, and the results are confirmed to be accurate by 
      LC-MS/MS; therefore, this method is valuable for the quality control of 
      animal-derived food.
FAU - Xiao, Jiaxu
AU  - Xiao J
AUID- ORCID: 0000-0001-9132-4294
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Wei, Nana
AU  - Wei N
AD  - Kunpeng Institute of Modern Agriculture at Foshan, Foshan 528200, China.
FAU - Wu, Shuangmin
AU  - Wu S
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Li, Huaming
AU  - Li H
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Yin, Xiaoyang
AU  - Yin X
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Si, Yu
AU  - Si Y
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Li, Long
AU  - Li L
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
FAU - Peng, Dapeng
AU  - Peng D
AD  - National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key 
      Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong 
      Agricultural University, Wuhan 430070, China.
AD  - Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, 
      Shenzhen 518000, China.
AD  - Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, 
      Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural 
      Sciences, Shenzhen 518000, China.
AD  - Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics 
      Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, 
      China.
LA  - eng
GR  - No.2020BBB079/The Key Research and Development Program of Hubei Province (CN) 
      (No.2020BBB079)/
GR  - 2662022DKPY007/The Fundamental Research Funds for the Central Universities 
      (2662022DKPY007)/
GR  - SZYJY2022024/The HZAU-AGIS Cooperation Fund (SZYJY2022024)/
PT  - Journal Article
DEP - 20220729
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antibodies)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/analysis
MH  - Antibodies/analysis
MH  - *Biosensing Techniques
MH  - Chromatography, Liquid/methods
MH  - Humans
MH  - Milk/chemistry
MH  - *Pork Meat
MH  - *Red Meat/analysis
MH  - Swine
MH  - Tandem Mass Spectrometry/methods
PMC - PMC9405744
OTO - NOTNLM
OT  - aminoglycosides
OT  - antibody chip
OT  - cephalosporins
OT  - multi-residues
OT  - sulfonamides
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/26 06:00
MHDA- 2022/08/27 06:00
PMCR- 2022/07/29
CRDT- 2022/08/25 07:24
PHST- 2022/07/05 00:00 [received]
PHST- 2022/07/25 00:00 [revised]
PHST- 2022/07/27 00:00 [accepted]
PHST- 2022/08/25 07:24 [entrez]
PHST- 2022/08/26 06:00 [pubmed]
PHST- 2022/08/27 06:00 [medline]
PHST- 2022/07/29 00:00 [pmc-release]
AID - bios12080578 [pii]
AID - biosensors-12-00578 [pii]
AID - 10.3390/bios12080578 [doi]
PST - epublish
SO  - Biosensors (Basel). 2022 Jul 29;12(8):578. doi: 10.3390/bios12080578.