PMID- 36006340
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 9
IP  - 8
DP  - 2022 Aug 11
TI  - Epidemiological Survey of Four Reproductive Disorder Associated Viruses of Sows 
      in Hunan Province during 2019-2021.
LID - 10.3390/vetsci9080425 [doi]
LID - 425
AB  - Porcine reproductive disorders have been considered as the major factors that 
      threaten pig industries worldwide. In this study, 407 aborted-fetus samples were 
      obtained from 89 pig farms in Hunan province, to investigate the prevalence of 
      four viruses associated with porcine reproductive disease, including porcine 
      reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 
      (PCV2), pseudorabies virus (PRV), and classical swine fever virus (CSFV). 
      Meanwhile, the target gene sequences of representative PRRSV (ORF5), PCV2 (ORF2), 
      CSFV (E2), and PRV (gE) strains were amplified, sequenced, and analyzed. The 
      results showed that the positive rates of PRRSV, PCV2, PRV, and CSFV among the 
      collected samples were 26.29% (107/407), 52.83% (215/407), 6.39% (26/407), and 
      12.29% (50/407), respectively. Moreover, co-infection with two and three 
      pathogens were frequently identified, with PCV2/PRRSV, PRRSV/CSFV, PRRSV/PRV, 
      PCV2/CSFV, PCV2/PRV, and PRRSV/PCV2/CSFV mix infection rates of 9.09%, 3.19%, 
      2.95%, 3.69%, 2.21%, and 0.49%, respectively. Moreover, ORF5-based phylogenetic 
      analysis showed that 9, 4, and 24 of 37 PRRSV strains belonged to the PRRSV2 
      lineages 1, 5, and 8, respectively. ORF2-based phylogenetic analysis revealed 
      that PCV2d and PCV2b were prevalent in Hunan province, with the proportions of 
      87.5% (21/24) and 12.5% (3/24), respectively. An E2-based phylogenetic tree 
      showed that all 13 CSFV strains were clustered with 2.1 subgenotypes, these 
      isolates were composed of 2.1b (10/13) and 2.1c (3/13) sub-subgenotypes. A 
      gE-based phylogenetic tree showed that all six PRV strains belonged to the 
      genotype II, which were genetically closer to variant PRV strains. Collectively, 
      the present study provides the latest information on the epidemiology and 
      genotype diversity of four viruses in sows with reproductive diseases in Hunan 
      province, China, which would contribute to developing effective strategies for 
      disease control.
FAU - Tang, Qiwu
AU  - Tang Q
AD  - Hunan Biological and Electromechanical Polytechnic, Changsha 410128, China.
FAU - Ge, Lingrui
AU  - Ge L
AD  - Hunan Biological and Electromechanical Polytechnic, Changsha 410128, China.
FAU - Tan, Shengguo
AU  - Tan S
AD  - Hunan Biological and Electromechanical Polytechnic, Changsha 410128, China.
FAU - Zhang, Hai
AU  - Zhang H
AD  - Animal Epidemic Prevention Station of Xiangxi Autonomous Prefecture, JiShou City 
      416000, China.
FAU - Yang, Yu
AU  - Yang Y
AD  - Animal Disease Prevention and Control Center, Wangcheng District, Changsha 
      410128, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Subdistrict Office of Nanzhuangping Street, Yongding District, Zhangjiajie 
      427000, China.
FAU - Deng, Zaofu
AU  - Deng Z
AD  - Hunan Biological and Electromechanical Polytechnic, Changsha 410128, China.
LA  - eng
GR  - 17JZ17/Zaofu Deng/
GR  - 20TD06/Zaofu Deng/
GR  - 2021NK4231/Qiwu Tang/
PT  - Journal Article
DEP - 20220811
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC9416293
OTO - NOTNLM
OT  - Hunan province
OT  - epidemiology
OT  - genotype diversity
OT  - reproductive disorder associated viruses
OT  - sow
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/26 06:00
MHDA- 2022/08/26 06:01
PMCR- 2022/08/11
CRDT- 2022/08/25 10:42
PHST- 2022/07/13 00:00 [received]
PHST- 2022/07/26 00:00 [revised]
PHST- 2022/08/08 00:00 [accepted]
PHST- 2022/08/25 10:42 [entrez]
PHST- 2022/08/26 06:00 [pubmed]
PHST- 2022/08/26 06:01 [medline]
PHST- 2022/08/11 00:00 [pmc-release]
AID - vetsci9080425 [pii]
AID - vetsci-09-00425 [pii]
AID - 10.3390/vetsci9080425 [doi]
PST - epublish
SO  - Vet Sci. 2022 Aug 11;9(8):425. doi: 10.3390/vetsci9080425.